Open Access Highly Accessed Research article

A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

Deepak Prem, María-Teresa Solís, Ivett Bárány, Héctor Rodríguez-Sanz, María C Risueño and Pilar S Testillano*

Author Affiliations

Plant Development and Nuclear Architecture, Centro de Investigaciones Biológicas, CIB-CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain

For all author emails, please log on.

BMC Plant Biology 2012, 12:127  doi:10.1186/1471-2229-12-127

Published: 2 August 2012

Additional files

Additional file 1:

In vivomicrospore gametophytic development inBrassica napus. Developmental stages from donor plants grown under low temperature conditions in growth chambers. Semi-thin sections stained with toluidine blue showing the structural organization of the young microspore (a), vacuolated microspore (b) and bicellular pollen (c). Squash preparations of anthers stained with DAPI and revealing the nuclei of others microspores and pollen at the same developmental stages, young microspore (a’), vacuolated microspore (b’) and bicellular pollen (c’). (PDF 32 kb)

Format: PDF Size: 32KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Statistical analysis of the contribution of several factors to embryo production in microspore cultures. 2 × 2 factor ANOVA for partitioning variance for microspore embryogenesis response of buds collected from donor plants grown in two different conditions and after isolation, subjected to two different thermal stress conditions (8 replicate data). (PDF 67 kb)

Format: PDF Size: 68KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Statistical analysis of the contribution of several factors to microspore embryo germination. 2 × 2 × 3 factorial ANOVA for partitioning variation in response of embryo conversion to plantlets (germination) for microspore embryos obtained from two different pathways and from donor plants of growth chambers, cultured directly or air-desiccated and subjected to three different germination pre-treatments. (4 replicate each with 7–20 embryos cultured per replicate for each factor). (PDF 39 kb)

Format: PDF Size: 39KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data