Figure 5 .
Assessment of OsMT1e-P transgenic tobacco plants for their tolerance towards various abiotic stresses and heavy metal toxicity. (A) Floating leaf disc senescence assay for abiotic stress tolerance in transgenic tobacco plants (T2). Experiments were performed on three transgenic lines (L1, L2 and L7) subjected to either 420 C for heat stress, 10 mM CuSO4, 10 mM ZnSO4, 40 C for cold stress, 5% PEG or 200 mM NaCl stress in Hoagland medium. Heat stress was given for 8 h only while other stresses were given for 5 days. Leaf discs floated in Hoagland medium served as the experimental controls. (B) Histogram depicting chlorophyll content retained in corresponding leaf discs shown in A. The data represent mean ± SE of three biological replicates (n = 3). Bars with different letters are statistically significant and those with same letter are not significantly different (p < 0.05).
Kumar et al. BMC Plant Biology 2012 12:107 doi:10.1186/1471-2229-12-107