Figure 1 .
Graphical (scaled) representation of location of putative genes for OsMTs on O. sativa chromosomes and qRT PCR analysis of clustered MT genes localized on chromosome XII. (A) The centromeres on chromosomes have been marked in circles. The position of first exon of genes (in Mb) has been marked in the parentheses along with their names at same location on chromosomes. Arrow marks the direction of the ORF specific to the OsMT genes. Enlarged view of a portion of chromosome XII (inset) shows a cluster of MT genes and their distribution revealing the striking similarity in the gene structure of the members. Intron/exon(s) are shown as thin and thick line, respectively. (B) Real-time PCR analysis of OsMT1a, OsMT1c, and OsMT1d expression in shoots of 4-day old rice seedlings under various abiotic stresses such as salinity (200 mM NaCl), drought (air drying) and ABA (100µM) for various durations i.e. 8 h or 24 h. Expression of various MTs have been shown relative to that of the MT1d which is the lowest expressed gene under control conditions. The controls were grown in half strength Yoshida medium. Data are means ± SE. Each data set represents an average of minimum three separate experiments.
Kumar et al. BMC Plant Biology 2012 12:107 doi:10.1186/1471-2229-12-107