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Open Access Research article

Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination

Huan Xia13, Marna Yandeau-Nelson24, Donald B Thompson3 and Mark J Guiltinan4*

Author Affiliations

1 MARS Petcare US, 315 Cool Springs Boulevard, Franklin, Tennessee 37067, USA

2 Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, Iowa 50011-3260, USA

3 Department of Food Science, The Pennsylvania State University, University Park, Pennsylvania 16802-2504, USA

4 Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802-5807, USA

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BMC Plant Biology 2011, 11:95  doi:10.1186/1471-2229-11-95

Published: 21 May 2011

Additional files

Additional file 1:

Chromatograms of isoamylase-debranched amylopectin and amylose fractions from Wt (----) and sbe1a mutant (- - -) starch.

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Open Data

Additional file 2:

Difference plots between sbe1a mutant and Wt starch for the proportions of chains from debranched β-dextrins during time course of β-amylolysis of amylopectin. Individual plots for sbe1a mutant and Wt are presented in Figure 1A.

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Open Data

Additional file 3:

Chain length distribution of isoamylase-debranched and isoamylase-plus-pullulanase-debranched β-limit dextrins from the amylopectin fraction from Wt and sbe1a mutant starch.

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Open Data

Additional file 4:

Chain length distribution of isoamylase-debranched and isoamylase-plus-pullulanase-debranched β-limit dextrins from the amylose fraction from Wt and sbe1a mutant starch.

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Open Data

Additional file 5:

Bright field (left) and polarized light (right) micrographs of residual starch after 16 h α-amylase digestion from Wt and sbe1a mutant. Arrows point to residual granules with dark center.

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Open Data

Additional file 6:

Transmission electron micrographs of residual starch after 16 h α-amylase digestion from Wt (left) and sbe1a mutant (right). Scale bars represent 5 μm at the top of the graphs.

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Open Data

Additional file 7:

Chromatograms of isoamylase-debranched resistant starch from Wt (----) and sbe1a mutant (- - -) starch

Format: PDF Size: 27KB Download file

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Open Data