Figure 7.

Regeneration of transformed coffee plants from maintained embryogenic cultures. Calli in selective media containing 100 mg.L-1hygromycin and 125 mg.L-1 cefotaxime 4 months after co-cultivation with A. tumefaciens LBA1119 without plasmid, used as control (A). Regeneration of resistant calli in selective media containing 100 mg.L-1hygromycin and 125 mg.L-1 cefotaxime 4 months after co-cultivation with A. tumefaciens LBA1119 carrying pMDC32; the yellow calli are resistant to hygromycin (B). Regeneration of torpedo-shaped somatic embryos 6 months after co-cultivation (C). In vitro plantlet development 8 months after co-cultivation (D). Transgenic plants in the greenhouse 12 months after co-cultivation (E).

Ribas et al. BMC Plant Biology 2011 11:92   doi:10.1186/1471-2229-11-92
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