Table 3

Multiplexes of polymorphic loci (with their allele size range in bp) for characterizing the Mediterranean olive tree germplasm *.

Multiplex PCR

Locus no.

Allele size range (bp)

Multiplex PCR

Locus no.

Allele size range (bp)



A-1 (NED-M13)

46

110-112

B-2 (HEX-M13)

48

158-159

1

121-124

25

174-177

9

135-136

36

182-183

51

139-146

52

191-203

22

158-159

58

234-236

41

169-171

C-1 (FAM-M13)

21

103-104

A-2 (NED-M13)

17

178-179

38

109-111

28

182-183

31

131-133

56

188-190

15

137-138

53

203-204

47

154-157

50

227-228

59

164-165

33

235-236

C-2 (FAM-M13)

6

173-174

B-1 (HEX-M13)

39

105-106

49

181-182

27

112-113

24

187-189

23

120-121

29

203-204

11

126-136

57

224-227

42

137-139

54

231-239

2

148-150


* After PCR, the six multiplex PCRs (35 loci) were mixed together with locus 10 (allele size range of 87 to 95 bp) and ROX 500 as internal standard, and then run on an ABI Prism 3100 Genetic Analyzer.

Besnard et al. BMC Plant Biology 2011 11:80   doi:10.1186/1471-2229-11-80

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