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Open Access Research article

Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana

Sigrid Husar1, Franz Berthiller2, Shozo Fujioka3, Wilfried Rozhon1, Mamoona Khan1, Florian Kalaivanan1, Luisa Elias4, Gillian S Higgins4, Yi Li4, Rainer Schuhmacher2, Rudolf Krska2, Hideharu Seto3, Fabian E Vaistij4, Dianna Bowles4 and Brigitte Poppenberger14*

Author Affiliations

1 Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria

2 Center for Analytical Chemistry, Department of Agrobiotechnology, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430 Tulln, Austria

3 RIKEN Advanced Science Institute, Wako-shi, Saitama 351-0198, Japan

4 Center for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, UK

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BMC Plant Biology 2011, 11:51  doi:10.1186/1471-2229-11-51

Published: 24 March 2011

Abstract

Background

Brassinosteroids (BRs) are signaling molecules that play essential roles in the spatial regulation of plant growth and development. In contrast to other plant hormones BRs act locally, close to the sites of their synthesis, and thus homeostatic mechanisms must operate at the cellular level to equilibrate BR concentrations. Whilst it is recognized that levels of bioactive BRs are likely adjusted by controlling the relative rates of biosynthesis and by catabolism, few factors, which participate in these regulatory events, have as yet been identified. Previously we have shown that the UDP-glycosyltransferase UGT73C5 of Arabidopsis thaliana catalyzes 23-O-glucosylation of BRs and that glucosylation renders BRs inactive. This study identifies the closest homologue of UGT73C5, UGT73C6, as an enzyme that is also able to glucosylate BRs in planta.

Results

In a candidate gene approach, in which homologues of UGT73C5 were screened for their potential to induce BR deficiency when over-expressed in plants, UGT73C6 was identified as an enzyme that can glucosylate the BRs CS and BL at their 23-O-positions in planta. GUS reporter analysis indicates that UGT73C6 shows over-lapping, but also distinct expression patterns with UGT73C5 and YFP reporter data suggests that at the cellular level, both UGTs localize to the cytoplasm and to the nucleus. A liquid chromatography high-resolution mass spectrometry method for BR metabolite analysis was developed and applied to determine the kinetics of formation and the catabolic fate of BR-23-O-glucosides in wild type and UGT73C5 and UGT73C6 over-expression lines. This approach identified novel BR catabolites, which are considered to be BR-malonylglucosides, and provided first evidence indicating that glucosylation protects BRs from cellular removal. The physiological significance of BR glucosylation, and the possible role of UGT73C6 as a regulatory factor in this process are discussed in light of the results presented.

Conclusion

The present study generates essential knowledge and molecular and biochemical tools, that will allow for the verification of a potential physiological role of UGT73C6 in BR glucosylation and will facilitate the investigation of the functional significance of BR glucoside formation in plants.

Keywords:
arabidopsis; brassinosteroids; glycosylation; homeostasis; malonylation; steroids