Skip to main content
  • Research article
  • Open access
  • Published:

Structure, expression differentiation and evolution of duplicated fiber developmental genes in Gossypium barbadense and G. hirsutum

Abstract

Background

Both Gossypium hirsutum and G. barbadense probably originated from a common ancestor, but they have very different agronomic and fiber quality characters. Here we selected 17 fiber development-related genes to study their structures, tree topologies, chromosomal location and expression patterns to better understand the interspecific divergence of fiber development genes in the two cultivated tetraploid species.

Results

The sequence and structure of 70.59% genes were conserved with the same exon length and numbers in different species, while 29.41% genes showed diversity. There were 15 genes showing independent evolution between the A- and D-subgenomes after polyploid formation, while two evolved via different degrees of colonization. Chromosomal location showed that 22 duplicate genes were located in which at least one fiber quality QTL was detected. The molecular evolutionary rates suggested that the D-subgenome of the allotetraploid underwent rapid evolutionary differentiation, and selection had acted at the tetraploid level. Expression profiles at fiber initiation and early elongation showed that the transcripts levels of most genes were higher in Hai7124 than in TM-1. During the primary-secondary transition period, expression of most genes peaked earlier in TM-1 than in Hai7124. Homeolog expression profile showed that A-subgenome, or the combination of A- and D-subgenomes, played critical roles in fiber quality divergence of G. hirsutum and G. barbadense. However, the expression of D-subgenome alone also played an important role.

Conclusion

Integrating analysis of the structure and expression to fiber development genes, suggests selective breeding for certain desirable fiber qualities played an important role in divergence of G. hirsutum and G. barbadense.

Background

Cotton (Gossypium spp.) is the world's most important fiber crop plant. While most of the > 50 Gossypium species are diploid (n = 13), five are allopolyploids (n = 26), originating from an interspecific hybridization event between A- and D-genome diploid species. Humans have independently domesticated four different species for their fiber, two of which are diploids, Gossypium herbaceum and G. arboreum, and two are allopolyploids, G. hirsutum and G. barbadense [1].

Alhough G. hirsutum and G. barbadense probably originated from a single hybridization event between A- and D- diploid species, the two have very different agronomic and fiber quality characteristics. The high yield potential and diverse environmental and production system adaptability of G. hirsutum make it the most widely cultivated species, accounting for about 97% of the world's cotton fiber [2]. G. barbadense is a more modern species possessing superior fiber quality. Novel alleles are responsible for the improved fiber quality in G. barbadense. Despite its higher fiber quality, however, the narrow adaptation range and low yield of G. barbadense limit its cultivation. The two Gossypium species are sexually compatible, although partial sterility, longer maturity, and hybrid breakdown are often observed in later generations [3]. Nonetheless, the introgression of favorable alleles from G. barbadense to G. hirsutum would likely improve the fiber quality of G. hirsutum while simultaneously maintaining its high fiber yield [4].

The cotton fiber is a single cell without the complex cell division and multicellular development that develops from ovule's epidermal cells. Fiber development occurs in four distinct, but overlapping stages: initiation, elongation, secondary wall synthesis, and maturation [5]. To date, many of the genes predominantly expressed in cotton fiber development have been isolated and characterized. Gh14-3-3L was found to be predominantly expressed during early fiber development, and may be involved in regulating fiber elongation [6]. Yoder et al. [7] defined pectate lyase (PEL) as a cell wall modification enzyme. GhPel was found to play an essential role in fiber cell elongation by degradation of the de-esterified pectin for cell wall loosening [8]. Ruan et al. [9] suggested the sucrose synthase gene (Sus) played an important role in the initiation and elongation of single-celled fibers by influencing carbon partitioning to cellulose synthesis. GhBG (β-1,4-glucosidase), one of three cellulases, was specifically expressed in fiber cells and plays an important role in degradation of the primary cell wall and promotion of secondary cell wall synthesis [10]. Cotton CelA1 and CelA2 genes, encoding the catalytic subunit of cellulose synthase, are expressed at high levels during active secondary wall cellulose synthesis in developing cotton fibers [11]. Two cotton Rac genes, GhRacA and GhRacB, expressed in the fibers at the initiation and elongation stages, might play an important role in early fiber development [12]. In addition, several genes are expressed specifically or preferentially in fibers [13–18], although their exact functional roles remain unclear.

In theory, there are two homologs in tetraploid cotton species, representing descendants from the A-genome and D-genome donors at the time of polyploidy formation. The goals of this study were to: 1) better understand the genetic basis of cotton fiber development, 2) identify the structural difference of duplicated genes, and 3) reveal the expression and evolution of fiber quality differences between upland and sea-island cotton. To complete this study, we selected 17 fiber development genes accessioned in National Center for Biotechnology Information (NCBI, http://www.ncbi.nlm.nih.gov) to study structure and expression differences of the two cultivated tetraploid species. To investigate their frame and sequence divergence, we initially cloned these genes in the genome DNA of the G. hirsutum accession TM-1, the G. barbadense cultivar Hai7124, and their two putative diploid progenitors. The chromosomal locations of each homeolog of several studied genes, having effective single nucleotide polymorphism (SNP) or amplification polymorphism loci between TM-1 and Hai7124 were determined by linkage analysis in allotetraploid cotton using an interspecific BC1 mapping population (TM-1×Hai7124)×TM-1] [19–22]. Finally, expression patterns of each gene and each homeolog were explored. A more thorough understanding of interspecific divergence of cotton will provide a solid foundation from which key fiber quality genes may be exploited in cotton molecular breeding.

Results

Sequence and structure analysis of fiber development genes

The orthologs of each of the 17 genes were cloned and sequenced (GenBank accession numbers (GQ340731-GQ340736, HQ142989-HQ143048, and HQ143055-HQ143090; Table 1). Phylogenetic groupings and sequence comparisons allowed the copy number for all genes, except Exp1 in the Hai7124 cultivar, to be independently isolated with a single copy from the diploid species and two homeologs from each tetraploid species for each gene. There was a single copy of Exp1 in the diploid species and two distinct copies in TM-1, however, the Exp1 sequence from Hai7124 was of only one type, though more than 10 clones were selected randomly to sequence. This result was further validated by a different primer pair of this gene (see Additional file 1: Supplemental Table S1 for list of primer pairs). The sequence from Hai7124 has a closer relationship with G. raimondii than with G. herbaceum. Further, southern blotting of Exp1 performed on the four species, showed two distinct hybridizing bands after digestion with EcoRI and HindI in Hai7124 and TM-1, and one hybridizing band in G. herbaceum and G. raimondii (Figure not shown), which indicated that Exp1 had two copies in both TM-1 and Hai7124. Combining sequence and southern blot analysis, the homeolog of Exp1 in the A-subgenome of Hai7124 was colonized to a type resembling that of the D-subgenome via nonreciprocal homoeologous exchange [23].

Table 1 Names and characteristics of fiber development-related genes

The lengths of the genomic DNA sequences isolated from the four species varied from 1020 bp (Exp1) to 6126 bp (CelA3) (Table 2). Based on the alignments between the genomic DNA and the cDNA sequences in orthologs, twelve genes (70.59%) shared the same intron/exon structures in different genomes, and variations in length were mainly caused by insertion/deletion events within introns (Additional file 2: Supplemental Figure S1A). The remaining five genes (29.41%), CIPK1, CAP, BG, ManA2 and CelA3, produced some structure differences caused by different exon length or numbers (Additional file 2: Supplemental Figure S1B).

Table 2 Structure analysis for orthologs of fiber development genes in four cotton species

Seventeen gene trees were constructed using the NJ method to distinguish the duplicated genes independent of evolution or local interlocus recombination after tetraploid formation. Two major clades, one including G. herbaceum and the A-subgenomes of TM-1 and Hai7124, the other including G. raimondii and the D-subgenomes of TM-1 and Hai7124, were formed for 15 genes (Additional file 3: Supplemental Figure S2A). High bootstrap values supported duplicated genes independent of evolution after tetraploid formation. Two genes were determined to have local interlocus recombination or colonization after tetraploid formation (Additional file 3: Supplemental Figure S2B). ACT1 from G. raimondii was more closely related to ACT1s from G. herbaceum and the A-subgenomes than with ACT1s from the D-subgenomes. This relationship suggests that ACT1s from the D-subgenomes evolved at an accelerated rate, relative to ACT1s from the A-subgenomes. The Exp1 sequence in Hai7124 was closer to that found in G. raimondii.

To locate all 17 homeolog gene pairs on our backbone genetic map [23], the subgenome-specific PCR primers (Additional file 4: Supplemental Table S2) and single-nucleotide amplified polymorphisms (SNAP) primers (Additional file 5: Supplemental Table S3) were used to detect polymorphisms between TM-1 and Hai7124. Polymorphic primer pairs were also used to survey 138 individuals of the ([(TM-1×Hai7124)×TM-1]) BC1 mapping population (Table 3). Eight gene pairs were located on their corresponding homeologous chromosomes, and each of six pairs were located on one of their homeologous chromosomes, while three pair of genes, Exp, Exp1 and CelA1 could not be mapped because no available polymorphic loci were found between TM-1 and Hai7124. A large body of data was compiled by integrating previously reported cotton fiber quality quantitative trait locus (QTL)[24–34] with the 22 identified fiber quality-related genes within 20 cM. Most genes had at least one fiber quality QTL; some had several (Table 3), indicating important fiber quality roles.

Table 3 Integration analysis of chromosomal locations of genes and fiber quality QTL reported in other studies

Rates of sequence evolution

With only a few exceptions, purifying selection, as indicated by Ka/Ks < 1, appears to be in place for most of pairwise comparisons (Table 4). The exceptions include G. herbaceum (A1 genome, A1) vs G. hirsutum A-subgenome (Ath) of POD2 and D5 vs Dth of RacA, which demonstrated positive selection (Ka/Ks > 1), as well as D5 vs Dtb of RacA, A1 vs Atb of 14-3-3L, A1 vs Ath and A1 vs Atb of CAP, which had exceptionally strong positive selection (Ka/Ks >> 1).

Table 4 Synonymous and nonsynonymous substitution rates in various comparisons among different cotton species

Because there were no nucleotide substitutions in A1 vs Atb of RacA, A1 vs Atb of EXP, D5 vs Dth and D5 vs Dtb of LTP3, and all RacBs pairs, it was not possible to compare their evolutionary rates. Although those particular "not applicable" comparisons (NA vs NA) were excluded from our analysis, the "NAs" were considered zero when they were compared with others whose Ks were not NA. In all pairwise comparisons of nucleotide diversity for each gene between subgenomes within a species [32 pairs, 16 in TM-1 and 16 in Hai7124 (RacB was excluded)], 62.5% (10 in TM-1 and 10 in Hai7124) had a higher evolutionary rate in the D-subgenome than in the A-subgenome. Furthermore, in the 16 gene pairs (excluding RacB) from the A-subgenomes of TM-1 and Hai7124, 62.5% (10 out of 16) had a higher evolutionary rate in TM-1 than in Hai7124, 31.25% (5 of 15) and were reversed and 6.25% (1 of 16, ACT1) showed an equivalent evolutionary rate between TM-1 and Hai7124. Similarly, in the 15 gene pairs (RacB and LTP3 were excluded) from the D-subgenomes of TM-1 and Hai7124, 60% (9 of 15) had a higher evolutionary rate in TM-1 than in Hai7124, 26.67% (4 of 15) and were reversed and 13.33% (2 of 15, Pel and Exp) showed an equivalent evolutionary rate between TM-1 and Hai7124.

Phylogenetic relationships are reflected in the nucleotide substitution results (Additional file 3: Supplemental Figure S2). Based on branch length, all of homeologs from the two tetraploid species had unequal rates of sequence evolution following allopolyploid formation. The rates at which the deviations occurred in allopolyploids are sufficient to generate branch length inequality between the A- and D-subgenomes [35].

Ka/Ks ratio comparisons showed that selection had altered the molecular evolutionary rate of some genes due to allopolyploid formation. Four genes, Pel, RacA, Exp and Sus1, in TM-1, and five genes, Pel, CIPK1, 14-3-3L, CAP and CelA3, in Hai7124, yielded higher Ka/Ks ratios in A-At, D-Dt and At-Dt comparisons than in the A-D comparison, indicating that selection for some genes related with fiber development had acted at the tetraploid level.

Differential expression fiber development genes

After the specificity of homeolog-specific primer pairs were confirmed by PCR amplification of genomic DNA from G. herbaceum (A-genome), G. raimondii (D-genome), TM-1 and Hai7124 (Figure 1), their homeolog transcripts in young tetraploid cotton fiber were further detected by qPCR analysis. The relative expression values at 10 different fiber development stages were obtained by combining the homeolog transcripts of each gene at the same stage. Expression for the 17 genes could be broken down into five categories (Additional file 6: Supplemental Figure S3): 1) fiber initiation and early elongation (0-8 DPA), such as Exp, POD2 and ManA2 (Additional file 6: Supplemental Figure S3A); 2) fiber elongation (3-17 DPA), such as Exp1, Pel, and LTP3 (Additional file 6: Supplemental Figure S3B); 3) primary-secondary transition period (17-23 DPA), such as BG, CEL and CelA1 (Additional file 6: Supplemental Figure S3C); 4) both at fiber initiation and early elongation period (0-8DPA) and secondary cell wall thickening period (20-23DPA), such as Sus1, 14-3-3L and RacB (Additional file 6: Supplemental Figure S3D); 5) the whole fiber developmental period, such as CelA3, CAP, ACT1, RacA and CIPK1 (Additional file 6: Supplemental Figure S3E). In the last category, however, transcript preference was shown at some stages. For example, CelA3 and CAP were expressed preferentially at the fiber elongation and secondary cell wall thickening stages (8-23 DPA), but had moderate expression at 0-5 DPA.

Figure 1
figure 1

Amplification products in four cotton species using subgenome-specific qPCR primer pairs. First line includes amplified results from A-genome specific primers; second line includes amplified results from D-genome specific primers. "M" represents marker, "A" represents G. herbaceum var. africanum, "D" represents G. raimondii, "T" represents G. hirsutum acc. TM-1, "H" represents G. barbadense cv. Hai7124. Numbers represent the sizes of the makers (bp).

Gene expression differences in TM-1 and Hai7124 were further clarified by statistical analysis of least signification difference (LSD). Greater expression in Hai7124 than in TM-1 was observed for 14-3-3L except at 20 DPA, and for CelA3 except at 5, 17 and 23 DPA. Other gene transcripts showed different expression advantages in the two cotton species at various fiber developmental stages.

At fiber initiation and early elongation (0-8 DPA), most genes, including Exp, ManA2, Sus1, RacB, CelA3, CAP and RacA, had significantly higher expression levels in Hai7124 than in TM-1. During fiber elongation (5-14 DPA), the expression profiles of genes preferentially expressed during that period were either biased to TM-1 or Hai7124 or were equally expressed between the two. Five genes, Exp1, Pel, CAP, CIPK1 and RacA, were expressed preferentially in TM-1 or equally between TM-1 and Hai7124, except at 8 DPA, that these same genes showed significantly greater expression levels in Hai7124; LTP3 and ACT1 showed significantly higher expression levels in TM-1 than in Hai7124; expression of CelA3 was higher in Hai7124.

During primary-secondary cell wall transition (17-23 DPA), peak expression occurred earlier in TM-1 than in Hai7124 for most genes (CAP, CelA3, CIPK1, CEL, BG, RacB, Sus1 and 14-3-3L). ACT1 and RacA expressed equally in TM-1 and Hai7124 at 17 and 20 DPA, but significantly greater in Hai7124 at 23 DPA. The extended fiber development period, as indicated by higher expression at a later DPA, may help explain why G. barbadense has an extra long staple cotton. One gene, CelA1, showed no significant expression difference between TM-1 and Hai7124.

Genome-specific expression of the homeologs

Based on the homeolog expression profile, 17 diagnostic genes in TM-1 and 17 in Hai7124 were further evaluated. Of the 34 genes, 32.35% (11) were equally expressed between the A- and D-subgenomes, 41.18% (14) were A-subgenome biased, 20.59% (7) were D-subgenome biased and 5.88% (2) were A- or D-biased at different stages.

The 17 fiber development genes were clustered into three comparison patterns between TM-1 and Hai7124. First, homeologs for CelA3, Exp, Exp1 and CIPK1 in both TM-1 and Hai7124 were equally expressed between the A- and D-subgenomes in the preferentially-expressed stages (Figure 2). Of these, Exp1 had equal transcript levels from the two homeologs in TM-1 and Hai7124, with two distinguishable copies in TM-1 and two undistinguishable copies in Hai7124. These data were consistent with the fact that the duplicated loci for Exp1 in Hai7124 had the same sequence as the D-subgenome (Figure 2).

Figure 2
figure 2

Q-PCR analysis for homeologous expression of genes expressed equally between A- and D-subgenomes. Significant values were obtained by comparison between the two subgenomes. * P < 0.05, ** P < 0.01. See Table 2 for abbreviation designations. Vertical bars represented standard deviation (STD).

Second, the transcripts of 11 genes, CEL, Pel, Sus1, 14-3-3L, RacA, CelA1, ManA2, RacB, CAP, LTP3 and POD2, were A- or D-subgenome biased (Figure 3). Among these, CEL, Pel, Sus1, 14-3-3L and RacA were A-subgenome biased and CelA1, ManA2 and RacB were D-subgenome biased in both TM-1 and Hai7124 at all stages. The transcripts of the homeologs of CAP, LTP3 and POD2 were significantly altered in the preferentially expressed stages in TM-1 and Hai7124. In TM-1, the transcripts of CAP and LTP3 were significantly A-subgenome biased. However, the transcripts of the two genes in Hai7124 were equivalently expressed at most stages, only D-subgenome bias in LTP3 in the primary-secondary cell wall transition period detected. Expression of POD2 was A-subgenome biased at 0, 3 and 10 DPA and D-subgenome biased at 1 DPA in TM-1. In Hai7124, POD2 expression was A-subgenome biased at 0, 3 and 10 DPA and D-subgenome biased at 1, 5 and 8 DPA.

Figure 3
figure 3

Q-PCR analysis for homeologous expression of genes with A or D-subgenome biased expression. Significant values and vertical bars were same with Figure 2.

Third, BG was significantly (P < 0.001) affected only from the A-subgenome, and ACT1 was significantly (P < 0.001) affected from the D-subgenome at all stages in both TM-1 and Hai7124 (Figure 4). Based on the comparison patterns and the structural analysis of the two genes, we proposed that the homeolog of BG from the D-subgenome might be silenced and that of ACT1 from the A-subgenome may have novel roles in other species (neofunctionalization).

Figure 4
figure 4

Q-PCR analysis for homeologous expression of genes with subgenome-specific expression. Significant values and vertical bars were same with Figure 2.

Differences between TM-1 and Hai7124 in transcriptome contributions of the subgenome at key fiber developmental stages were detected. During initiation and early elongation of the fiber, 10 gene transcriptions showed greater expression levels in Hai7124 than in TM-1. Of those, the D-subgenome contributed higher amounts of ACT1, RacB and Man2, while the A-subgenome contributed higher amounts of Sus1, CIPK1 and 14-3-3L. CelA3, Exp, CAP and RacA were equally supplied by both subgenomes.

At 8 DPA, corresponding to the close of fiber plasmodesmate [36], the transcriptions of 12 genes (Exp1, Pel, POD2, CelA3, BG, Sus1, CAP, Exp, RacA, RacB, 14-3-3L and CIPK1) were sharply accumulated in Hai7124. Of those, the transcripts of RacB and POD2 were contributed mainly from D-subgenome, that of BG, Pel, Sus1 and RacA from A-subgenome and others by both A- and D-subgenomes.

At the primary-secondary transition period, the expression of 10 genes, CelA3, CAP, ACT1, RacA, CIPK1, 14-3-3L, Sus1, RacB, CEL and BG, occurred earlier in TM-1 than in Hai7124. The transcripts of ACT1 and RacB were mainly from D-subgenomes; those of BG, CEL and Sus1 were mainly from A-subgenomes, and other genes were from both A- and D-subgenomes. Based on these data we inferred that the expression accumulation of the A-subgenome, or the combination of A- and D-subgenomes, played critical roles in fiber quality divergence of G. hirsutum and G. barbadense. However, the expression of D-subgenome alone also played an important role.

Discussion

Evolutionary fate of duplicated genes

For each gene that was studied, allopolyploid species should have two homelogs, representing descendants from the A-genome and D-genome donors at the time of polyploidy formation. Cronn et al. [35] indicated that most duplicated genes in allopolyploid cotton evolved independently of each other. Our phylogenetic analyses support this hypothesis, and the independent evolution of several genes was distinctively evident in their structure, in our study. For example, CAPs and RacBs had the same structure between each diploid and its counterpart in allopolyploid cotton (A- and At-subgenome, D- and Dt-subgenome), but the different structures were apparent in the A-D comparison (Additional file 2: Supplemental Figure S1B). Though expression of ManA2 from the At-subgenome of Hai7124 ceased rather early in the growth process, the structure difference between the A-, At-subgenomes and D-, Dt-subgenomes was also distinct. The fact that the structure of the At- and Dt-subgenomes mirrored their putative ancestral diploid species suggested the difference may have occurred before allopolyploid formation and evolved independently in allopolyploid cotton. CelA3s from At-subgenome of TM-1 and Hai7124 displayed the same mutation, which altered their coding regions, indicating not only independent evolution, but also parallel evolution between TM-1 and Hai7124. This change, however, was not detected in their putative ancestral diploid species, suggesting accelerated evolution of CelA3 in the At-subgenome after allopolyploid formation. Though most genes independently evolved in allopolyploid cotton, there were some exceptions. For example, Exp1 from At-subgenome were colonized in Hai7124 by Dt-subgenomes.

Relative to expression, duplicate genes can follow one of three evolutionary paths. First, one copy may evolve into a nonfunctional pseudogene [37–41]. Second, the multiple copies can contribute to an increase in the gene expression level [42, 43] or both copies can suffer mutations but the combined action of both gene copies is necessary to maintain original function and expression levels (subfunctionalization) [40, 44, 45]. Third, one copy may gain a novel beneficial function (neofunctionalization) that is selectively maintained within the genome [40, 46–48]. We measured homeolog-specific contributions to the transcriptome in allopolyploid cotton fiber by Q-PCR analysis. Because the majority (64.70%) of diagnostic genes exhibited subgenome-specific bias to the A or D-subgenome, subgenome-biased expression in cotton fiber developmental stages was considered commonplace. This result was consistent with previous studies [49–55]. Most of genes in our study exhibited the same expression bias in the two cultivated cotton species, TM-1 and Hai7124. However, some inconsistencies were detected in three genes (CAP, LTP3 and POD2), suggesting that these genes may have had different roles in the interspecific divergence between G. hirsutum and G. barbadense. Artificial selection by humans of certain desirable fiber traits may have also influenced G. hirsutum and G. barbadense genetic structure [55].

Synthesizing structures and expression profiles of the duplicates, their possible fates are inferred. BG accumulated solely in A-subgenome transcripts (D-subgenome silenced), in both TM-1 and Hai7124 (Figure 4). BGs obtained from the D-subgenomes of TM-1 and Hai7124 had a nucleotide deletion and a nonsense mutation, respectively, which altered the ORFs (Table 2). The structure difference suggests that BG in the D-subgenomes of TM-1 and Hai7124 may be pseudogenes. On the other hand, while CAP and CelA3 had different A- and D-subgenome structures in both TM-1 and Hai7124 (Additional file 2: Supplemental Figure S1B), their A- and D-subgenome expression profiles were active (Figure 2, 3). Therefore, the duplicated genes of CAP and CelA3 may be subfunctionalized. Similarly, the functions of duplicated genes from CEL, Sus1, 14-3-3L, RacA, RacB, Exp, Exp1, CIPK1, CelA1, Pel, ManA2, LTP3 and POD2 were also subfunctionalized (Figure 3). Because ACT1 transcripts of A subgenomes could not be detected at all stages of fiber development (Figure 4), they may have evolved new functions.

Domestication of allopolyploid cotton

Numerous plant species have been selectively bred over the course of human social evolution [56]. Allopolyploid cotton species are believed to have formed about 1-2 million years ago, by hybridization between a maternal Old World diploid A-genome G. herbaceum [57] and paternal New World diploid D-genome G. raimondii [57–59]. The allotetraploid lineage gave rise to five extant tetraploid species, including G. barbadense and G. hirsutum, known for their superior fiber quality and high yield, respectively. In the present study, the Ka/Ks ratios among four cotton species indicated that selection of fiber development genes occurred at the tetraploid level. By comparing the nucleotide diversity between TM-1 and Hai7124 within the same subgenome, most genes (62.5% in A-subgenome and 60% in D-subgenome) had a higher evolutionary rate in TM-1 than in Hai7124, which may be associated with longer and more frequent cultivation of TM-1. Given these data, we propose that diversity evolution between A- and D-subgenomes within a species or between TM-1 and Hai7124 within the same subgenome was due to both natural and artificial selection pressure [55].

Gene expression differences between TM-1 and Hai7124

G. hirsutum and G. barbadense are two domesticated cotton species possessing very different agronomic and fiber quality characteristics with G. barbadense having superior fiber quality. Rapp et al. (2010) studied the transcriptomes of cotton fibers from wild and domesticated accessions (G. hirsutum) and found that human selection during the initial domestication and subsequent crop improvement had resulted in a biased upregulation of components of the transcriptional network during fiber development [60]. In this study, of the 17 fiber development-related genes, 14 had the similar expression pattern and three that did not, in TM-1 and Hai7124 (Figure 2, 3, 4). Of three genes, the transcripts of homeologs were significantly A- or D-subgenome biased in TM-1. However, in Hai7124, homeolog transcripts were equally expressed between the two subgenomes or D-subgenome biased. Though 14 genes had the same expression patterns between TM-1 and Hai7124, the relative expression levels were different at most stages. While the same A- or D-biased or equal expression profile in the two cultivated cotton species might be related to functional partitioning of genomic contributions during cellular development after allopolyploid formation, significant alternation of homoelog A/D ratio and expression difference at the same fiber developmental time points between G. hirsutum and G. barbadense indicated that domestication for different fiber qualities may play an important role in fiber quality divergence of G. hirsutum and G. barbadense.

In previous study, fiber growth curves have shown longer fiber elongation phases in domesticated G. hirsutum than that in wild G. hirsutum, and further comparative gene expression profiling of isolated cotton fibers over a developmental time course of fiber differentiation indicated that domesticated TM-1 displayed a much higher level of transcriptional variation between the sampled time points than the wild accession did [60]. In the study, the expression peak of transcripts in most genes was earlier in TM-1 than in Hai7124, especially at the primary-secondary transition period, which indicated that most genes related to fiber development expressed longer and more intensely in Hai7124. Resulting differences in mRNA levels may lead to changes in enzyme activity, further contributing to phenotypic differences between the two cotton species. Several genes that are differentially expressed in TM-1 and Hai7124 should be further mined.

The 14-3-3 protein is an important regulatory protein. Shi et al. [6] proposed that the Gh14-3-3L transcripts are highly accumulated during early cotton fiber development, suggesting that Gh14-3-3L may be involved in regulating fiber elongation. Our data showed that, although 14-3-3L is expressed preferentially in the early development stages of cotton fibers in both TM-1 and Hai7124, the relative expression values were significantly different. The expression of 14-3-3L was significantly higher in most stages in Hai7124 (Additional file 6: Supplemental Figure S3E), than in TM-1. Furthermore in the primary-secondary transition period of fiber development, a secondary expression peak of 14-3-3L was also detected, indicating 14-3-3L may also be involved in thickening of the secondary cell wall, a function that has been reported in other species [61]. At 23 DPA, the expression of 14-3-3L decreased in TM-1, but was still increasing in Hai7124, suggesting a longer transcriptional period.

CelA1 was solely expressed during secondary wall cellulose synthesis, with no significant expression difference between TM-1 and Hai7124 (Additional file 6: Supplemental Figure S3C). The importance of CelA genes in secondary wall cellulose synthesis in developing cotton fibers has been reported by other researchers [16]. Expression of CelA3 at 17 DPA was significantly higher in TM-1 than in Hai7124. However, strength dropped much faster in TM-1 (e.g., at 20 DPA, Additional file 6: Supplemental Figure S3E) than in Hai7124 after that, which more stable expression was detected over a longer period of time in Hai7124.

Rac genes (coding for GTP-binding Rac proteins) are involved in various physiological processes, including cell polar growth [62–65], synthesis of the secondary wall [66–68], resistance response [69, 70] and signal transduction [71–73]. In the present study, RacAs were predominantly expressed at the fiber elongation and secondary wall synthesis stages (Additional file 6: Supplemental Figure S3E), indicating that the role of RacA may be similar to that of Rac13, which is involved in the signal transduction pathway for cytoskeleton organization [66]. Similarly, RacB was predominantly expressed at the secondary wall synthesis stage in TM-1, and preferentially expressed at the initiation of fiber development and secondary wall synthesis in Hai7124 (Additional file 6: Supplemental Figure S3D), suggesting multiple roles for RacB. Because RacA and RacB expressions were consistently higher at most stages in Hai7124 relative to TM-1, the two genes may be contributing to fiber quality diversity.

Sucrose synthase (Sus) affects initiation and elongation of the single-celled fibers, and also has a major role in cell wall cellulose synthesis [9, 74–77]. Cotton fiber cell initiation and elongation are very sensitive to changes in Sus activity [9]. The expression peak of cotton sucrose synthase genes transcripts was earlier in wild cotton than in TM-1[60]. Our study showed that greater Sus1 expression in Hai7124 than in TM-1 at initiation (0 and 1 DPA) and 8 DPA and at 3 and 5 DPA was reversed. At 20 DPA, Sus1 expression was significantly higher in TM-1, possibly as a result of earlier termination of fiber elongation and earlier initiation of cell wall synthesis in TM-1.

Recent studies of comparative gene expression profiling of isolated cotton fibers [60, 78] have identified genes controlling differences in fiber growth between wild and domesticated cotton [e.g., genes encoding tubulin isoforms, endotransglycosylase/hydrolases, cytochrome P(450) monooxygenase, and antioxidant enzymes]. These studies will provide new clues in the future studies to better understand the interspecific divergence of fiber development in the two cultivated tetraploid cotton species, G. barbadense and G. hirsutum.

Putative role of D-subgenome in interspecific divergence

Most genes (62.5% in TM-1 and 62.5% in Hai7124) exhibited a higher evolutionary rate in the D-subgenome than in the A-subgenome, indicating that the D-subgenome of the allotetraploid evolved faster than the A-subgenome [59, 79–82]. Considering the weight of evidence provided by genome-specific expression of homeologs, chromosome location of genes and QTL distribution for fiber qualities (Table 3), we also determined the subgenome transcriptome contribution in interspecific divergence between TM-1 and Hai7124. In the integration interval of genes and QTLs, the gene transcripts were significantly A-subgenome biased, and the QTLs associated with fiber qualities were detected in corresponding A-subgenome chromosome bins. Similarly, the gene transcripts were significantly D-subgenome biased and relative fiber QTLs were detected in corresponding D-subgenome chromosome bins. While the transcripts of CAP was significantly A-subgenome biased in TM-1, but equally expressed in Hai7124 in the fiber developmental period, implying an important D-subgenome contribution for elite fiber quality traits in Hai7124. Integration of CAP duplicated loci with fiber length and strength QTLs in D13, indicates domestication and artificial selection of tetraploid plants for superior fiber quality resulted in more significant evolutionary dynamics in the D-subgenome than in the A-subgenome.

Conclusion

The study provided us the systematic report on the interspecific divergence for fiber development between Gossypium barbadense and G. hirsutum by analyzing structures, molecular evolution and transcripts levels of fiber development-related genes. The results indicated that selective breeding for certain desirable fiber qualities may have played an important role in divergence of the two cultivated tetraploid cotton species.

Methods

Plant materials

Of the 17 genes selected for this study (Table 1) were described in previous reports as being expressed in developing cotton fibers; their sequence information was deposited in GenBank. The orthologous loci of each of the 17 genes were isolated from two allotetraploid cottons, G. hirsutum acc. TM-1, G. barbadense cv. Hai7124, and living models of their two ancestral genome donors, G. herbaceum var. africanum (A-genome) and G. raimondii Ulbrich (D-genome). For expression analysis, developing cotton ovules and attached fibers were harvested from three replicates of TM-1 and Hai7124 on 0, 1, 3, 5, 8, 10, 14, 17, 20 and 23 days post-anthesis (DPA). The mapping population was comprised of 138 BC1 individuals generated from the cross [(TM-1×Hai7124) ×TM-1] [19].

PCR amplification, cloning and sequencing

Based on GenBank deposited sequences, gene-specific PCR primer pairs were individually designed for PCR-amplification of full-length sequences of the 17 genes. For the smaller genes, we designed one primer pair; at least two nested primer pairs were designed for the larger genes (Additional file 1: Supplemental Table S1).

Standard polymerase chain reaction (PCR) was completed using High-fidelity ExTaq DNA polymerase (TaKaRa Biotechnology (Dalian) Co., Ltd., China). The PCR products were cloned into pMD18-T Vector (TaKaRa) according to the manufacturer's instructions, and sequenced from plasmid DNA templates. In order to obtain the sequence from both the A-subgenome and D-subgenome, at least 10 clones for each gene from each of the tetraploid species, TM-1 and Hai7124, were picked randomly and sequenced. Homeologs were identified by comparison to sequences from their diploid progenitors. To avoid possible complications originating from PCR recombination in allopolyploid cotton [83], a minimum of three clones was used to determine the gene sequence in each duplicated copy. In cases where PCR recombination occurred, 10 additional clones were sequenced to verify the corresponding correct sequence [83]. When the copy number of genes was difficult to identify by sequencing, it was confirmed by southern blot analysis.

Lengths were determined using alignment analysis, which was applied to each relevant sequence of applicable gene. For genes with one primer pair, the A- and D-subgenome sequences were clustered using the Neighbor-Joining method in MEGA3.1 http://www.megasoftware.net/. For those genes having more than one primer pair, sequence segments from the same genomes and subgenomes were contiged by CAP3 software.

Gene structure and phylogenetic analyses

Six DNA sequences for each gene, including homeologs and orthologs, isolated from the four species (A-, D-genome and the A and D subgenome from the allotetraploid), were aligned by ClustalX http://www.ebi.ac.uk/Tools/msa/clustalw2/. Based on the sequences of genomic DNA and the cDNA, the structure of each gene was illustrated by the Gene Structure Display Server (GSDS, http://gsds.cbi.pku.edu.cn/chinese.php). Phylogenetic analyses of the orthologs for each gene were performed using the Neighbor-Joining method in MEGA3.1 http://www.megasoftware.net/.

Chromosomal location

In order to locate all 17 pairs of homeologs on our genetic map, PCR primers were designed according to the sequence size difference between the two allotetraploid cottons (Additional file 4: Supplemental Table S2). For homeologs with no size polymorphism, single-nucleotide amplified polymorphism (SNAP) primers were designed based on a SNP between the TM-1 and Hai7124 sequences at a putative locus (Additional file 5: Supplemental Table S3). SNAP is a modified allele-specific amplified method, it introduce an additional base pair change in the primer to increase the specificity of the primer [84]. All SNAP primers was designed following Drenkard et al. [84] and using SNAPER http://ausubellab.mgh.harvard.edu.

All the primer pairs exhibiting polymorphisms between TM-1 and Hai7124 were used to survey 138 individuals of the BC1 mapping population. The polymorphic loci were integrated in our backbone map [22] using Joinmap 3.0 software [85].

Evolution and ratios of sequence forms

For each gene, synonymous substitution rates (Ks), nonsynonymous substitution rates (Ka) and their ratios (Ka/Ks) in various comparisons among the four species (A1 VS D5, A1 VS At, D5 VS Dt, At VS Dt) were calculated for the coding region using KaKs_Calculator http://sourceforge.net/projects/kakscalculator2/. Ks is the rate of evolution and the Ka/Ks ratio indicates the selective force acting on the protein [86]. A Ka/Ks ratio of 1 represents neutral evolution, that is, the number of nonsynonymous changes at each possible nonsynonymous site is the same as the number of synonymous changes per synonymous site. Ka/Ks < 1 indicates a purifying selection (selection generally eliminates deleterious mutations and maintains protein status quo); Ka/Ks > 1 indicates positive selection, where selection may be modifying the protein. The higher the ratio is, the stronger the evidence that selection is occurring.

Development of homeolog-specific PCR primer pairs

Homeolog-specific primers were designed and applied based on sequence differences between duplicated loci (Additional file 7: Supplemental Table S4). Specificity of those primers was detected by PCR amplification of genomic DNA from G. herbaceum (A-genome), G. raimondii (D-genome), TM-1 and Hai7124. Primers specific for the A-genome resulted in amplifications from G. herbaceum, TM-1 and Hai7124, but not from G. raimondii, which indicated that the amplification products of TM-1 and Hai7124 were from their A-subgenomes. Conversely, the D-genome specific primers only amplified from G. raimondii and D-subgenome of the allopolyploid.

RNA isolation and Q-PCR analysis

Total RNA was extracted from young fibers of G. hirsutum acc. TM-1 and G. barbadense cv. Hai7124 at 0, 1, 3, 5, 8, 10, 14, 17, 20 and 23 DPA using the CTAB-acid phenol extraction method [87]. RNA samples were treated with DNase I (Ambion, Austin, TX, USA) according to manufacturer's instructions to remove trace contaminants of genomic DNA. Total RNA samples (1 μg per reaction) were reversely transcribed into cDNAs by avian myeloblastosis virus (AMV) reverse transcriptase, and the cDNAs were used as templates in Q-PCR reactions.

For each gene, the homeolog-specific primers were designed based on the SNP between A- and D-subgenome homeologs (Additional file 7: Supplemental Table S4). A cotton elongation factor (EF1a) gene was used as a standard control in the Q-PCR reactions (Additional file 7: Supplemental Table S4). The Q-PCR reaction and calculated relative value for expression level of each homeolog were determined as described by Wang et al. [8]. The information of the Q-PCR analysis based on the MIQE checklist was submitted in Additional file 8.

Abbreviations

SNP:

Single Nucleotide Polymorphism

SNAP:

Single-Nucleotide Amplified Polymorphisms

QTL:

Quantitative Trait Loci.

References

  1. Brubaker CL, Bourland FM, Wendel JF: The origin and domestication of cotton. Cotton: origin, history, technology and production volume 1. Edited by: Smith WC, Cothren TJ. New York: John Wiley and Sons; 1999: 3-31. 1

    Google Scholar 

  2. NCCoA: World cotton database. 2006, National Cotton Council of America, [http://www.cotton.org/econ/cropinfo/cropdata/index.cfm]

    Google Scholar 

  3. Stephens SG: The genetics of ''Corky''. The New World alleles and their possible role as an interspecific isolating mechanism. J Genet. 1946, 47: 150-161. 10.1007/BF02986784.

    Article  PubMed  CAS  Google Scholar 

  4. Chee P, Draye X, Jiang CX, Decanini L, Delmonte TA, Bredhauer R, Smith CW, Paterson AH: Molecular dissection of interspeciffic variation between Gossypium hirsutum and G. barbadense (cotton) by a backcross-self approach: I. Fiber elongation. Theor Appl Genet. 2005, 111: 757-763. 10.1007/s00122-005-2063-z.

    Article  PubMed  CAS  Google Scholar 

  5. Basra AS, Malik CP: Development of the cotton fiber. Int Rev Cytol. 1984, 89: 65-113. full_text.

    Article  CAS  Google Scholar 

  6. Shi HY, Wang XL, Li DD, Tang WK, Wang H, Xu WL, Li XB: Molecular characterization of cotton 14-3-3L gene preferentially expressed during fiber elongation. J Genet and Genomics. 2007, 34: 151-159. 10.1016/S1673-8527(07)60016-2.

    Article  CAS  Google Scholar 

  7. Yoder MD, Keen NT, Jurnak F: New domain motif: the structure of pectate lyase c, a secreted plant virulence factor. Science. 1993, 260: 1503-1507. 10.1126/science.8502994.

    Article  PubMed  CAS  Google Scholar 

  8. Wang HH, Guo Y, Lv FN, Zhu HY, Wu SJ, Jiang YJ, Li FF, Zhou BL, Guo WZ, Zhang TZ: The essential role of GhPEL gene, encoding a pectate lyase in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton. Plant Mol Biol. 2010, 72: 397-406. 10.1007/s11103-009-9578-7.

    Article  PubMed  CAS  Google Scholar 

  9. Ruan YL, Llewellyn DJ, Furbank RT: Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell. 2003, 15: 952-964. 10.1105/tpc.010108.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  10. Ma GJ, Zhang TZ, Guo WZ: Cloning and characterization of cotton GhBG gene encoding B-glucosidase. DNA Sequence. 2006, 17: 355-362.

    Article  PubMed  CAS  Google Scholar 

  11. Pear JR, Kawagoe Y, Schreckengost WE, Delmer DP, Stalker DM: Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase. Proc Natl Acad Sci USA. 1996, 93: 12637-12642. 10.1073/pnas.93.22.12637.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  12. Li XB, Xiao YH, Luo M, Hou L, Li DM, Luo XY, Pei Y: Cloning and expression analysis of two Rac genes from cotton (Gossypium hirsutum L.). Acta Genetica Sinica. 2005, 32: 72-78.

    PubMed  Google Scholar 

  13. Li XB, Fan XP, Wang XL, Cai L, Yang WC: The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. Plant Cell. 2005, 17: 859-875. 10.1105/tpc.104.029629.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  14. Orford SJ, Timmis JN: Specific expression of an expansin gene during elongation of cotton fibers. Biochin Biophy Acta. 1998, 1398: 342-346.

    Article  CAS  Google Scholar 

  15. Laosinchai W, Cui X, Brown RM: A full cDNA of cotton cellulose synthase has high homology with the Arabidopsis RSW1 gene and cotton CelA1 (Accession No. AF 200453) (PGR 00-002). Plant Physiol. 2000, 122: 291-10.1104/pp.122.1.291.

    Article  Google Scholar 

  16. Cui X, Shin H, Song C, Laosinchai W, Amano Y, Brown RM: A putative plant homolog of the yeast beta-1, 3-glucan synthase subunit FKS1 from cotton (Gossypium hirsutum L.) fibers. Planta. 2001, 213: 223-230. 10.1007/s004250000496.

    Article  PubMed  CAS  Google Scholar 

  17. Gao P, Zhao PM, Wang J, Wang HY, Wu XM, Xia GX: Identification of genes preferentially expressed in cotton fibers: A possible role of calcium signaling in cotton fiber elongation. Plant Sci. 2007, 173: 61-69. 10.1016/j.plantsci.2007.04.008.

    Article  CAS  Google Scholar 

  18. He YJ, Guo WZ, Shen XL, Zhang TZ: Molecular cloning and characterization of a cytosolic glutamine synthetase gene, a fiber strength-associated gene in cotton. Planta. 2008, 228: 473-483. 10.1007/s00425-008-0751-z.

    Article  CAS  Google Scholar 

  19. Song XL, Wang K, Guo WZ, Zhang J, Zhang TZ: A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L.×G. barbadense L. Genome. 2005, 48: 378-390. 10.1139/g04-126.

    Article  PubMed  CAS  Google Scholar 

  20. Han ZG, Guo WZ, Song XL, Zhang TZ: Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton. Mol Genet Genomics. 2004, 272: 308-327. 10.1007/s00438-004-1059-8.

    Article  PubMed  CAS  Google Scholar 

  21. Han ZG, Wang CB, Song XL, Guo WZ, Guo JY, Li CH, Chen XY, Zhang TZ: Characteristics, development and mapping of Gossypium hirsutum derived EST-SSR in allotetraploid cotton. Theor Appl Genet. 2006, 112: 430-439. 10.1007/s00122-005-0142-9.

    Article  PubMed  CAS  Google Scholar 

  22. Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ: A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics. 2007, 176: 527-541. 10.1534/genetics.107.070375.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  23. Armel S, Flagel L, Ying B, Udall JA, Wendel JF: Homoeologous nonreciprocal recombination in polyploid cotton. New Phytologist. 2010, 186: 123-134. 10.1111/j.1469-8137.2009.03093.x.

    Article  Google Scholar 

  24. Frelichowski JE, Palmer MB, Main D, Tomkins JP, Cantrell RG, Stelly DM, Yu J, Kohel RJ, Ulloa M: Cotton genome mapping with new microsatellites from Acala 'Maxxa' BAC-ends. Mol Gen Genomics. 2006, 275: 479-491. 10.1007/s00438-006-0106-z.

    Article  CAS  Google Scholar 

  25. He DH, Lin ZX, Zhang XL, Nie YC, Guo XP, Zhang YX, Li W: QTL mapping for economic traits based on a dense genetic map of cotton with PCR-based markers using the interspecific cross of Gossypium hirsutum × Gossypium barbadense. Euphytica. 2007, 153: 181-197. 10.1007/s10681-006-9254-9.

    Article  CAS  Google Scholar 

  26. Lacape JM, Nguyen TB, Courtois B, Belot JL, Giband M, Gourlot JP, Gawryziak G, Roques S, Hau B: QTL analysis of cotton fiber quality using multiple Gossypium hirsutum × Gossypium barbadense backcross generations. Crop Sci. 2005, 45: 123-140.

    Article  CAS  Google Scholar 

  27. Lin Z, He D, Zhang X, Nie Y, Guo X, Feng C, Stewart JM: Linkage map construction and mapping QTL for cotton fiber quality using SRAP, SSR and RAPD. Plant Breeding. 2005, 124: 180-187. 10.1111/j.1439-0523.2004.01039.x.

    Article  CAS  Google Scholar 

  28. Luan M, Guo X, Zhang Y, Yao J, Chen W: QTL mapping for agronomic and fiber traits using two interspecific chromosome substitution lines of upland cotton. Plant Breding. 2009, 128: 671-679. 10.1111/j.1439-0523.2009.01650.x.

    Article  CAS  Google Scholar 

  29. Qin HD, Guo WZ, Zhang YM, Zhang TZ: QTL mapping of yield and fiber traits based on a four-way cross population in Gossypium hirsutum L. Theor Appl Genet. 2008, 117: 883-894. 10.1007/s00122-008-0828-x.

    Article  PubMed  Google Scholar 

  30. Shen XL, Guo WZ, Zhu XF, Yuan YL, Yu JZ, Kohel RJ, Zhang TZ: Molecular mapping of QTLs for fiber qualities in three diverse lines in Upland cotton using SSR markers. Mol Breeding. 2005, 15: 169-181. 10.1007/s11032-004-4731-0.

    Article  CAS  Google Scholar 

  31. Shen XL, Zhang TZ, Guo WZ, Zhu XF, Zhang XY: Mapping fiber and yield QTLs with main, epistatic, and QTL x Environment interaction effects in recombinant inbred lines of Upland cotton. Crop Sci. 2006, 46: 61-66. 10.2135/cropsci2005.0056.

    Article  CAS  Google Scholar 

  32. Shen XL, Guo WZ, Lu QX, Zhu XF, Yuan YL, Zhang TZ: Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in Upland cotton. Euphytica. 2007, 155: 371-380. 10.1007/s10681-006-9338-6.

    Article  CAS  Google Scholar 

  33. Wang BH, Guo WZ, Zhu XF, Wu YT, Huang NT, Zhang TZ: QTL mapping of fiber quality in an elite hybrid derived-RIL population of upland cotton. Euphytica. 2006, 152: 367-378. 10.1007/s10681-006-9224-2.

    Article  CAS  Google Scholar 

  34. Zhang ZS, Hu MC, Zhang J, Liu DJ, Zheng J, Zhang K, Wang W, Wan Q: Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.). Mol Breeding. 2009, 24: 49-61. 10.1007/s11032-009-9271-1.

    Article  Google Scholar 

  35. Cronn RC, Small RL, Wendel JF: Duplicated genes evolve independently following polyploid formation in cotton. Proc Natl Acad Sci USA. 1999, 96: 14406-14411. 10.1073/pnas.96.25.14406.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  36. Ruan YL, Lewellyn DJ, Furbank RT: The control of single-celled cotton fiber elongation by developmentally reversible gating of plasmadesmata and coordinated expression of sucrose and K+ transporters and expansin. Plant Cell. 2001, 13: 47-60. 10.1105/tpc.13.1.47.

    PubMed  CAS  PubMed Central  Google Scholar 

  37. Nei M, Gu X, Sitnikova T: Evolution by the birth-and-death process in multigene families of the vertebrate immune system. Proc Natl Acad Sci USA. 1997, 94: 7799-7806. 10.1073/pnas.94.15.7799.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  38. Petrov DA, Chao YC, Stephenson EC, Hartl DL: Pseudogene evolution in Drosophila suggest high rate of DNA loss. Mol Biol Evol. 1998, 15: 1562-1567.

    Article  PubMed  CAS  Google Scholar 

  39. Force A, Lynch M, Pickett FB, Amores A, Yan YL, Postlethwait J: Preservation of duplicate genes by complementary, degenerative mutations. Genetics. 1999, 151: 1531-1545.

    PubMed  CAS  PubMed Central  Google Scholar 

  40. Blumensteil JP, Hartl DL, Lozovsky ER: Patterns of insertion and deletion in contrasting chromatin domains. Mol Biol Evol. 2002, 19: 2211-2225.

    Article  Google Scholar 

  41. Lynch M, Conery JS: The origins of genome complexity. Science. 2003, 302: 1401-1404. 10.1126/science.1089370.

    Article  PubMed  CAS  Google Scholar 

  42. Kondrashov FA, Kondrashov AS: Role of selection in fixation of gene duplications. J Theor Biol. 2006, 239: 141-151. 10.1016/j.jtbi.2005.08.033.

    Article  PubMed  CAS  Google Scholar 

  43. Lynch M, Force A: The probability of duplicate-gene preservation by subfunctionalization. Genetics. 2000, 154: 459-473.

    PubMed  CAS  PubMed Central  Google Scholar 

  44. Lynch M, O'Hely M, Walsh B, Force A: The probability of preservation of a newly arisen gene duplicate. Genetics. 2001, 159: 1789-1804.

    PubMed  CAS  PubMed Central  Google Scholar 

  45. Clark AG: Invasion and maintenance of a gene duplication. Proc Natl Acad Sci USA. 1994, 91: 2950-2954. 10.1073/pnas.91.8.2950.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  46. Walsh JB: How often do duplicated genes evolve new fuctions?. Genetics. 1995, 139: 421-428.

    PubMed  CAS  PubMed Central  Google Scholar 

  47. Bergthorsson U, Andersson DI, Roth JR: Ohno's dilemma: evolution of new genes under continuous selection. Proc Natl Acad Sci USA. 2007, 104: 17004-17009. 10.1073/pnas.0707158104.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  48. Adams KL, Cronn R, Percifield R, Wendel JF: Genes duplicated by polyploidy show unequal contributions to the transcriptome and organ-specific reciprocal silencing. Proc Natl Acad Sci USA. 2003, 100: 4649-4654. 10.1073/pnas.0630618100.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  49. Adams KL, Percifield R, Wendel JF: Organ-specific silencing of duplicated genes in a newly synthesized cotton allotetraploid. Genetics. 2004, 168: 2217-2226. 10.1534/genetics.104.033522.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  50. Adams KL, Wendel JF: Allele-specific, bidirectional silencing of an alcohol dehydrogenase gene in different organs of interspecific diploid cotton hybrids. Genetics. 2005, 171: 2139-2142. 10.1534/genetics.105.047357.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  51. Udall JA, Swanson JM, Nettleton D, Percifield RJ, Wendel JF: A novel approach for characterizing expression levels of genes duplicated by polyploidy. Genetics. 2006, 173: 1823-1827. 10.1534/genetics.106.058271.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  52. Samuel Yang S, Cheung F, Lee JJ, Ha M, Wei NE, Sze SH, Stelly DM, Thaxton P, Triplett B, Town CD, Jeffrey Chen Z: Accumulation of genome-specific transcripts, transcription factors and phytohormonal regulators during early stages of fiber cell development in allotetraploid cotton. Plant J. 2006, 47: 761-775. 10.1111/j.1365-313X.2006.02829.x.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  53. Flagel L, Udall JA, Nettleton D, Wendel JF: Duplicate gene expression in allopolyploid Gossypium reveals two temporally distinct phases of expression evolution. BMC Biol. 2008, 6: 16-24. 10.1186/1741-7007-6-16.

    Article  PubMed  PubMed Central  Google Scholar 

  54. Hovav R, Chaudhary B, Udall JA, Flagel L, Wendel JF: Parallel domestication, convergent evolution and duplicated gene recruitment in allopolyploid cotton. Genetics. 2008, 179: 1725-1733. 10.1534/genetics.108.089656.

    Article  PubMed  PubMed Central  Google Scholar 

  55. Chaudhary B, Flagel L, Stupar RM, Udall JA, Verma N, Springer NM, Wendel JF: Reciprocal silencing, transcriptional bias and functional divergence of homeologs in polyploid cotton (Gossypium). Genetics. 2009, 182: 503-517. 10.1534/genetics.109.102608.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  56. Raven P, Evert R, Eichhorn S: Biology of Plants. New York: Worth Publishers press: 1992.

    Google Scholar 

  57. Endrizzi JE, Turcotte EL, Kohel RJ: Genetics, cytology, and evolution of Gossypium. Adv Genet. 1985, 23: 271-375. full_text.

    Article  Google Scholar 

  58. Wendel JF: New world tetraploid cottons contain Old World cytoplasm. Proc Natl Acad Sci USA. 1989, 86: 4132-4136. 10.1073/pnas.86.11.4132.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  59. Zhu HY, Zhang TZ, Yang LM, Guo WZ: EST-SSR sequences revealed the relationship of D-genome in diploid and tetraploid Species in Gossypium. Plant Sci. 2009, 176: 397-405. 10.1016/j.plantsci.2008.12.007.

    Article  CAS  Google Scholar 

  60. Rapp RA, Haigler CH, Flagel L, Hovav RH, Udall JA, Wendel JF: Gene expression in developing fibres of Upland cotton (Gossypium hirsutum L.) was massively altered by domestication. BMC Biology. 2010, 8: 139-10.1186/1741-7007-8-139.

    Article  PubMed  PubMed Central  Google Scholar 

  61. Voigt J, Frank R: 14-3-3 proteins are constituents of the insoluble glycoprotein framework of the chlamydomonas cell wall. Plant Cell. 2003, 15: 1399-1413. 10.1105/tpc.010611.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  62. Li H, Wu G, Ware D, Davis KR, Yang ZB: Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast. Plant Physiol. 1998, 118: 407-417. 10.1104/pp.118.2.407.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  63. Kost B, Lemiche E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH: Rac homologues and compartmentalized phosphatidylinositol 4, 52 bisphosphate act in a common pathway to regulate polar pollen tube growth. J Cell Biol. 1999, 145: 317-330. 10.1083/jcb.145.2.317.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  64. Molendijk AJ, Bischoff F, Rajendrakumar CSV, Friml J, Braun M, Gilroy S, Palme K: Arabidopsis thaliana Rop GTPase are localized to tips of root hairs and control polar growth. EMBO J. 2001, 20: 2279-2288. 10.1093/emboj/20.11.2779.

    Article  Google Scholar 

  65. Jones MA, Shen JJ, Fu Y, Li H, Yang ZB, Grierson CS: The Arabidopsis Rop-GTPase is a positive regulator of both root hair initiation and tip Growth. Plant Cell. 2002, 14: 763-776. 10.1105/tpc.010359.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  66. Delmer DP, Pear JR, Andrawis A, Stalker DM: Genes encoding small GTP-binding proteins analogous to mammalian rac are preferentially expressed in developing cotton fibers. Mol Gen Genet. 1995, 248: 43-51. 10.1007/BF02456612.

    Article  PubMed  CAS  Google Scholar 

  67. Potikha TS, Collins CC, Johnson DJ, Delmer DP, Levine A: The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers. Plant Physiol. 1999, 119: 849-858. 10.1104/pp.119.3.849.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  68. Nakanomyo I, Kost B, Chua NH, Fukuda H: Preferential and asymmetrical accumulation of a Rac small GTPase mRNA in differentiating xylem cells of Zinnia elegans. Plant Cell Physiol. 2002, 43: 1484-1492. 10.1093/pcp/pcf170.

    Article  PubMed  CAS  Google Scholar 

  69. Kawasaki T, Henmi K, Ono E, Hatakeyama S, Iwano M, Satoh H, Shimamoto K: The small GTP-binding protein rac is a regulator of cell death in plants. Proc Natl Acad Sci USA. 1999, 96: 10922-10926. 10.1073/pnas.96.19.10922.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  70. Ono E, Wong HL, Kawasaki T, Hasegawa M, Kodama O, Shimamoto K: Essential role of the small GTPase Rac in disease resistance of rice. Proc Natl Acad Sci USA. 2001, 98: 759-764. 10.1073/pnas.021273498.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  71. Li H, Shen JJ, Zheng ZL, Lin YK, Yang ZB: The Rop GTPase switch controls multiple developmental processes in Arabidopsis. Plant Physiol. 2001, 126: 670-684. 10.1104/pp.126.2.670.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  72. Tao LZ, Cheung AY, Wu HM: Plant Rac-like GTPases are activated by auxin and mediate auxin-responsive gene expression. Plant Cell. 2002, 14: 2745-2760. 10.1105/tpc.006320.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  73. Zheng ZL, Nafisi M, Arvin T, Li H, Crowell DN, Chary SN, Schroeder JI, Shen JJ, Yang ZB: Plasma membrane-associated ROP10 small GTPase is a specific negative regulator of abscisic acid responses in Arabidopsis. Plant Cell. 2002, 14: 2787-2797. 10.1105/tpc.005611.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  74. Amor Y, Haigler CH, Johnson S, Walnscott M, Delmer DP: A membrane-associated form of Sus and its potential role in synthesis of cellulose and callose in plants. Proc Natl Acad Sci USA. 1995, 92: 9353-9357. 10.1073/pnas.92.20.9353.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  75. Nolte KD, Hendrix DL, Radin JW, Koch KE: Sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules. Plant Physiol. 1995, 109: 1285-1293.

    PubMed  CAS  PubMed Central  Google Scholar 

  76. Ruan YL, Chourey PS, Delmer DP, Perez-Grau L: The differential expression of sucrose synthase in relation to diverse patterns of carbon portioning in developing cotton seed. Plant Physiol. 1997, 115: 375-385.

    PubMed  CAS  PubMed Central  Google Scholar 

  77. Ruan YL, Chourey PS: A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds. Plant Physiol. 1998, 118: 399-406. 10.1104/pp.118.2.399.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  78. Chaudhary B, Hovav R, Flagel L, Mittler R, Wendel JF: Parallel expression evolution of oxidative stress-related genes in fiber from wild and domesticated diploid and polyploidy cotton (Gossypium). BMC Genomics. 2009, 10: 378-10.1186/1471-2164-10-378.

    Article  PubMed  PubMed Central  Google Scholar 

  79. Small RL, Ryburn JA, Wendel JF: Low levels of nucleotide diversity at homoelogous Adh loci in allotetraploid cotton (Gossypium L.). Mol Biol Evol. 1999, 16: 491-501.

    Article  PubMed  CAS  Google Scholar 

  80. Liu Q, Brubaker CL, Green AG, Marshall DR, Sharp PJ, Singh SP: Evolution of the FAD2-1 fatty acid desaturase 5'UTR intron and the molecular systematics of Gossypium (Malvaceae). Am J Bot. 2001, 88: 92-102. 10.2307/2657130.

    Article  PubMed  CAS  Google Scholar 

  81. Small RL, Wendel JF: Differential evolutionary dynamics of duplicated paralogous Adh loci in alloteraploid cotton (Gossypium). Mol Biol Evol. 2002, 19: 597-607.

    Article  PubMed  CAS  Google Scholar 

  82. An C, Saha S, Jenkins JN, Ma DP, Scheffler BE, Kohel RJ, Yu JZ, Stelly DM: Cotton (Gossypium spp.) R2R3-MYB transcription factors SNP identification, phylogenomic characterization, chromosome localization, and linkage mapping. Theor Appl Genet. 2008, 116: 1015-1026. 10.1007/s00122-008-0732-4.

    Article  PubMed  CAS  Google Scholar 

  83. Cronn RC, Small RL, Haselkorn T, Wendel JF: Rapid diversification of the cotton genus (Gossypium: Malvaceae) revealed by analysis of sixteen nuclear and chloroplast gene. Am J Bot. 2002, 89: 707-725. 10.3732/ajb.89.4.707.

    Article  PubMed  CAS  Google Scholar 

  84. Drenkard E, Richter BG, Rozen S, Stutius LM, Angell NA, Mindrinos M, Cho RJ, Oefner PJ, Davis RW, Ausubel FM: A simple procedure for the analysis of single nucleotide polymorphisms facilitates Map-Based Cloning in Arabidopsis. Plant Physiol. 2000, 124: 1483-1492. 10.1104/pp.124.4.1483.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  85. Van-Ooijen JW, Vorrips RE: JoinMapR Version 3.0: Software for the Calculation of Genetic Linkage Maps. CPRO-DLO, Wageningen; 2001.

    Google Scholar 

  86. Hurst LD: The Ka/Ks ratio: diagnosing the form of sequence evolution. TRENDS in Genetics. 2002, 91: 8486-487.

    Google Scholar 

  87. Jiang JX, Zhang TZ: Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method. Cotton Sci. 2003, 15: 166-167.

    Google Scholar 

Download references

Acknowledgements

We thank RJ Kohel at U. S. Department of Agriculture-Agricultural Research Service, Southern Plains Agricultural Research Center, ZJ Chen at Institute for Cellular and Molecular Biology and Center for Computational Biology and Bioinformatics, University of Texas, and JE Zalapa at Department of Horticulture, University of Wisconsin for helpful comments and key editing. This program was financially supported in part by National Science Foundation in China (30730067), the State Key Basic Research and Development Plan of China (2011CB109300), Natural Science Foundation in Jiangsu Province (BK2008036), the National High-tech Program (2006AA10Z111), and the Program for 111 project (B08025).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Wangzhen Guo.

Additional information

Authors' contributions

Experiments were designed by WZG with suggestions from TZZ. WZG and HYZ conceived the experiments and analyzed the results. HYZ carried out most of the experiments and all computational analyses. Q-PCR Analysis was performed by XYH. JHL, LZ, and XYX participated in part of experiments. HYZ and WZG drafted the manuscript and TZZ revised the manuscript. All authors read and approved the final manuscript.

Electronic supplementary material

12870_2010_819_MOESM1_ESM.DOC

Additional file 1: Table S1. Primer pairs used for amplifying the full-length genomic sequences of each gene. (DOC 70 KB)

12870_2010_819_MOESM2_ESM.DOC

Additional file 2: Figure S1. Structure analysis of fiber development-related genes in four cotton species. A: G. herbaceum L. var. africanum; D: G. raimondii Ulbr; TM-1-At: A subgenome of G. hirsutum L. acc. TM-1; TM-1-Dt: D subgenome of G. hirsutum L. acc. TM-1; 7124-At: A subgenome of G. barbadense L. cv. 7124; 7124-Dt: D subgenome of G. barbadense L. cv. 7124. A. Orthologs having the same structures among four different cotton species. B. Orthologs having different structures among four different cotton species. (DOC 284 KB)

12870_2010_819_MOESM3_ESM.DOC

Additional file 3: Figure S2. The phylogenetic trees of 17 genes in four cotton species. Bootstrap values (%) based on 1000 replicates are indicated beside the nodes. A. Genes that evolved independently between the A- and D-subgenomes. B. Genes that evolved through different degrees of colonization between the A- and D-subgenome. (DOC 197 KB)

Additional file 4: Table S2. PCR primer pairs used for gene location. (DOC 36 KB)

Additional file 5: Table S3. SNP primer pairs used for gene location. (DOC 61 KB)

12870_2010_819_MOESM6_ESM.DOC

Additional file 6: Figure S3. Q-PCR analysis for total expression of genes. Significant differences between TM-1 and Hai7124 at the same stages indicated by * P > 0.05, ** P > 0.01. Vertical bars represented standard deviation (STD). A. Genes expressed preferentially at fiber initiation and early elongation (0-8 DPA). B. Genes expressed preferentially at fiber elongation (3-17 DPA). C. Genes expressed preferentially at primary-secondary transition (17-23 DPA). D. Genes expressed both at fiber initiation and early elongation period (0-8DPA) and secondary cell wall thickening period (20-23DPA). E. Genes expressed coving the whole fiber developmental period. (DOC 164 KB)

Additional file 7: Table S4. Subgenomic-specific PCR primer pairs used for Q-PCR analysis. (DOC 79 KB)

Additional file 8: The information of the Q-PCR analysis based on the MIQE checklist. (DOC 104 KB)

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Zhu, H., Han, X., Lv, J. et al. Structure, expression differentiation and evolution of duplicated fiber developmental genes in Gossypium barbadense and G. hirsutum. BMC Plant Biol 11, 40 (2011). https://doi.org/10.1186/1471-2229-11-40

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/1471-2229-11-40

Keywords