Additional file 2.

Figure S2-Antibody testing of anti-PHD5 and anti-ISWI. After solubilizing soybean leave nuclei in lysis buffer, the proteins were separated by 12% SDS-PAGE, and immunodetected with purified sera. A: The specificity of anti-PHD5 antibody was tested with recombinant GST-PHD5 by western blotting. B: The specificity of anti-PHD5 antibody was tested with soybean total proteins by western blotting. Anti-PHD5: anti-PHD5 antibody, Control: preimmune antiserum. C: The specificity of anti-ISWI antibody was tested with soybean total proteins by western blotting. Anti-ISWI: anti-ISWI antibody, Control: preimmune antiserum.

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Wu et al. BMC Plant Biology 2011 11:178   doi:10.1186/1471-2229-11-178