GmPHD5 could interact with GmISWI. The structure of GmISWI (see detail residues information in the text) and the two constructed vectors which were expressed in E. Coli (A). GST pull down assay indicated that GmISWI interacted with GmPHD5 through its N termini (B). The solubilized soybean nuclear proteins were immunoprecipitated with rabbit IgG, beads and anti-GmISWI antibody, respectively, followed by immunodetection using anti-GmPHD5 antibody (C). The solubilized soybean nuclear proteins were immunoprecipitated with anti-GmPHD5 antibody, rabbit IgG and beads, respectively, followed by immunodetected using anti-GmISWI antibody (C). All proteind were separated by 12% SDS-PAGE separation. These results are representative of three independent experiments.
Wu et al. BMC Plant Biology 2011 11:178 doi:10.1186/1471-2229-11-178