Figure 4.

Validation of the interaction between GmPHD5 and GmGNAT1, GmElongin A by GST pull down assay. Inputs of the GST pull down assay. Arrowheads pointed to the expressed protein MBP (upper), MBP-Elobgin (middle) and MBP GNAT1 (lower), respectively (A). Western blotting results of the GST pull down assay. Arrowheads pointed to the pulled down protein MBP-Elongin (upper) and MBP GNAT1 (lower), respectively (B). The diagram of the construction of the truncated GmPHD5. The N termini of the GmPHD5 without its PHD finger domain and the C termini of the GmPHD5 with only the PHD finger domain were inserted into the GST expression vector (C). GST pull down assay with the truncated GmPHD5. Arrowheads pointed to the pulled down protein MBP-Elongin (upper) and MBP GNAT1 (lower), respectively (D).

Wu et al. BMC Plant Biology 2011 11:178   doi:10.1186/1471-2229-11-178
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