Gametophyte development and cell isolation in Physcomitrella patens. (A) Plantlet on day 3 after protoplast formation (arrows indicate cell walls). (B) Plantlet on day 14 after protoplast formation. Ca = caulonema; Ch = chloronema. (C) Region of a plantlet on day 24 after protoplast formation (arrowheads show buds). (D) Plantlet on day 30 after protoplast formation. Plantlet contains leafy gametophores, protonema, and rhizoids (not visible in the image). (E-J) Images illustrating the process of laser-capture microdissection. Chloronema (E) and caulonema (H) before dissection. Arrowheads indicate cell walls between adjacent cells. Chloronema (F) and caulonema (I) after cell dissection and captured chloronemal (G) and caulonemal (J) cells. Bar = 50 μm in all panels.
Xiao et al. BMC Plant Biology 2011 11:177 doi:10.1186/1471-2229-11-177