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Open Access Highly Accessed Research article

Genome-wide transcriptome analysis of gametophyte development in Physcomitrella patens

Lihong Xiao1, Hui Wang1, Ping Wan1, Tingyun Kuang2 and Yikun He1*

Author Affiliations

1 College of Life Science, Capital Normal University, Beijing, 100048, P. R. China

2 Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, P. R. China

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BMC Plant Biology 2011, 11:177  doi:10.1186/1471-2229-11-177

Published: 15 December 2011

Additional files

Additional file 1:

Summary of Solexa tags in the cDNA libraries.

Format: XLS Size: 33KB Download file

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Additional file 2:

Distribution of distinct clean tags in each sample.

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Additional file 3:

Smear plots from the edgeR-based analysis of gene expression. Genes are plotted based on their log-fold change of transcript abundance between two compared samples on the y-axis and log concentration on the x-axis for raw tag libraries separately. Differentially expressed genes are shown in red.

Format: TIFF Size: 4.8MB Download file

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Additional file 4:

Complete list of transcripts attributed to different developmental stages and chloronemal or caulonemal tissues in 14-day-old protonema during P. patens gametophyte development.

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Additional file 5:

Gene ontology enrichment results for differentially expressed genes.

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Additional file 6:

The primer sequences of the randomly selected and internal genes used for real-time PCR analysis.

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Additional file 7:

Expression level of internal genes in each sample in the real-time PCR analysis.

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Additional file 8:

Complete list of each K-means cluster during gametophyte development in P. patens.

Format: XLS Size: 2MB Download file

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