TCL2 interacts with GL3 in plant cells and affects GL1 expression. A. TCL2 interacts with GL3 in plant cells. Reporter gene and effector genes were cotransfected into protoplasts derived from Arabidopsis rosette leaves. GUS activity was measured after 20-22 h incubation of the protoplasts in darkness. Data represent the mean ± SD of three replicates. Effectors and reporter constructs were diagrammed at the top of the figure. B. Regulation of GL1 by TCL2. RNA was isolated from 10-day-old seedlings of Col, 35S:HA-TCL2, 35S:HA-TCL1 and 35S:TCL2RNAi, and RT-PCR was used to examine the expression of GL1. Expression of ACT2 was used as a control.
Gan et al. BMC Plant Biology 2011 11:176 doi:10.1186/1471-2229-11-176