Open Access Open Badges Research article

Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening

Ana M Fortes1*, Patricia Agudelo-Romero1, Marta S Silva2, Kashif Ali3, Lisete Sousa4, Federica Maltese3, Young H Choi3, Jerome Grimplet5, José M Martinez- Zapater5, Robert Verpoorte3 and Maria S Pais1

Author Affiliations

1 Plant Systems Biology Lab, Departmento de Biologia Vegetal/ICAT, Center for Biodiversity, Functional and Integrative Genomics (BioFIG), FCUL, 1749-016 Lisboa, Portugal

2 Centro de Química e Bioquímica, Departamento de Química e Bioquímica, FCUL, Lisbon, Portugal

3 Natural Products Laboratory, Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

4 Department of Statistics and Operational Research, CEAUL (Centro de Estatística e Aplicações da UL), FCUL, Lisbon, Portugal

5 Instituto de Ciencias de la Vid y del Vino (CSIC, UR, Gobierno de La Rioja), CCT, C/Madre de Dios 51, 26006 Logroño, Spain

For all author emails, please log on.

BMC Plant Biology 2011, 11:149  doi:10.1186/1471-2229-11-149

Published: 2 November 2011

Additional files

Additional file 1:

Weather conditions from April to September in 2007 and 2008 seasons.

Format: XLSX Size: 16KB Download file

Open Data

Additional file 2:

Metabolism of organic acids and phenolic compounds. Relative quantification of tartaric acid, citric acid, acetic acid, cis-coumaroyl derivatives and trans-caftaric acid is based on characteristic chemical shift (δ 4.50, δ 2.93,, δ 1.91, δ 7.02 and δ 6, 38, respectively), and corresponding peak intensity. Expression levels of genes coding for Vitis vinifera L-idonate dehydrogenase (VVTU4643_at), and cinnamyl alcohol dehydrogenases (VVTU14855_at, VVTU21888_at, VVTU11923_at) was based on results of microarrays. *Accounts for a contamination of a spectrum corresponding to EL 32 sample collected in 2008 around δ 1.91.

Format: TIFF Size: 357KB Download file

Open Data

Additional file 3:

Wilcoxon Rank sum and Kruskal-Wallis statistics applied to metabolomics data.

Format: XLS Size: 503KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

COSY analysis in a sample from 2007 corresponding to EL 35 (véraison). Spectrum is shown in the range of δ 6.0 to δ 8.0 ppm which is enriched in phenolic compounds.

Format: JPEG Size: 815KB Download file

Open Data

Additional file 5:

Core set (7130 probesets) and conserved set (3451 probesets) of modulated genes during ripening. Information concerning fold change values, expression profile cluster, annotation, functional category and their distribution within clusters is provided.

Format: XLSX Size: 925KB Download file

Open Data

Additional file 6:

List of entire modulated gene set. Annotations based on the genome and based on EST-homology are provided. Separate lists of probesets differentially expressed at each year are included. All the information is made available in 6 sheets.

Format: XLS Size: 5.5MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 7:

List of primers used in real time reverse transcription-polymerase chain reaction.

Format: DOCX Size: 16KB Download file

Open Data