Figure 5.

Localization of DIR1 lacking its signal sequence. 35S:DIR1^Δ1-25-GUS-5/dir1-1 plants were left untreated (U), mock-inoculated (M) or induced for SAR with PstavrRpt2 (10⁶ cfu ml^-1). Inoculated (I) and systemic leaves (S) were collected from inoculated plants at 14 hpi. Leaves were stained for GUS activity and photographed in (A) or GUS activity levels were determined in the midveins, secondary veins and mesophyll cells of untreated (U), mock-inoculated (M) or leaves induced for SAR (I and S) in (B). The asterisk (*) denotes a significant difference (student's t test) between untreated and inoculated leaves from SAR-induced plants (I). This experiment was repeated once with similar results.