Fluorescence microscopy localization of caleosin in the olive anther. Sections from olive anthers at the PMC (A), Te (B), Mi (C), YP (D), MBP (E) and MP (F) stages were incubated with a FL anti-clo3 Ab, followed by an anti-rabbit IgG-Alexa Fluor 488-conjugated secondary Ab. Differential interference contrast (DIC) images of serial sections were also obtained to better visualize the different tissues of the anther. En: endothecium; Ep: epidermis; L: anther locule; MBP: mid bicellular pollen grain; Mi: microspore; MP: mature pollen grain; PMC: pollen mother cell; T: tapetum; Te: tetrad; YP: young pollen grain.
Zienkiewicz et al. BMC Plant Biology 2011 11:122 doi:10.1186/1471-2229-11-122