Table 2

A panel of meiotic genes and their expression patterns in ameiotic1 (am1) anthers.

Maize Gene ID

Names

Homolog

Stage

qRT Verified

Differentially Expressed


GRMZM2G075563

Ms5

plant only

various

down in am1-489 at 1.5 mm

GRMZM2G059037

Afd1

Rec8/Rad21

prophase I

down in am1-489 no change in am1-praI at both stages

GRMZM5G883855

Am1

plant only

pre-meiotic

down in am1-489; up in am1-praI at both stages

GRMZM2G109618

Dmc1

Dmc1

prophase I

down in both mutant alleles at 1.5 mm

GRMZM2G408897

Mmd1

plant only

early-late

down in both mutant alleles at 1.5 mm

GRMZM2G060394

Skp1A

Skp1A

prophase I

significantly down in both mutant alleles at 1.5 mm

GRMZM2G032562

Skp1B

Skp1B

significantly down in both mutant alleles at 1.5 mm

GRMZM2G143590

Zyp1

Zip1

prophase I

down in both mutant alleles at both stages

GRMZM2G083138

Rad54-like

Rad54-like

prophase I

down in both mutant alleles, particularly at 1.5 mm

GRMZM2G100103

Phs1

Rec114

prophase I

low or no expression in all

GRMZM2G129913

Spo11-1

Spo11

prophase I

nc

marginal changes

GRMZM2G454838/GRMZM2G328795

Zip4/Spo22-like

Zip4/Spo22-like

prophase I

down in both mutant

GRMZM5G883855

Aml1

Mei2

prophase I

no changes

GRMZM2G102242

Mnd1

Mnd1

prophase I

no changes

GRMZM2G308884

Prd1

Mei1

prophase I

no changes

GRMZM2G121543

Rad51A

Rad51

prophase I

no changes

GRMZM2G084762

Rad51A2

Rad51A2

prophase I

no changes

GRMZM2G115013

RPA70

RPA1a

various

down in am1-489 at 1.0 and 1.5 mm

GRMZM2G315902

Mlh3

Mlh3

prophase I

no changes or not significant

GRMZM2G055899

Pair1a

plant only

pre-meiotic

not significant

GRMZM2G056075

Msh5

Msh5

various

not significant except up in am1-489 at 1.5 mm

GRMZM2G457370

Ago1

Ago1

pre-meiotic

up in both mutant alleles at both stages


√: qRT analyses found the same direction of regulation and approximately the same magnitude of change as calculated from the microarray analysis; nc: qRT and array results were non-congruent.

Nan et al. BMC Plant Biology 2011 11:120   doi:10.1186/1471-2229-11-120

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