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Open Access Highly Accessed Database

SolRgene: an online database to explore disease resistance genes in tuber-bearing Solanum species

Vivianne GAA Vleeshouwers12*, Richard Finkers12, Dirk Budding1, Marcel Visser12, Mirjam MJ Jacobs12, Ralph van Berloo1, Mathieu Pel1, Nicolas Champouret1, Erin Bakker23, Pavel Krenek15, Hendrik Rietman1, DirkJan Huigen1, Roel Hoekstra24, Aska Goverse23, Ben Vosman12, Evert Jacobsen1 and Richard GF Visser12

Author Affiliations

1 Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

2 Centre for BioSystems Genomics, P.O. Box 98, 6700 AB, Wageningen, The Netherlands

3 Laboratory of Nematology, Wageningen University and Research Centre, Wageningen, The Netherlands

4 Centre for Genetic Resources, Wageningen University and Research Centre, Wageningen, The Netherlands

5 Centre of the Region Hana for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacky University, Slechtitelu 11, Olomouc, CZ-78371, Czech Republic

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BMC Plant Biology 2011, 11:116  doi:10.1186/1471-2229-11-116

Published: 18 August 2011

Abstract

Background

The cultivated potato (Solanum tuberosum L.) is an important food crop, but highly susceptible to many pathogens. The major threat to potato production is the Irish famine pathogen Phytophthora infestans, which causes the devastating late blight disease. Potato breeding makes use of germplasm from wild relatives (wild germplasm) to introduce resistances into cultivated potato. The Solanum section Petota comprises tuber-bearing species that are potential donors of new disease resistance genes. The aim of this study was to explore Solanum section Petota for resistance genes and generate a widely accessible resource that is useful for studying and implementing disease resistance in potato.

Description

The SolRgene database contains data on resistance to P. infestans and presence of R genes and R gene homologues in Solanum section Petota. We have explored Solanum section Petota for resistance to late blight in high throughput disease tests under various laboratory conditions and in field trials. From resistant wild germplasm, segregating populations were generated and assessed for the presence of resistance genes. All these data have been entered into the SolRgene database. To facilitate genetic and resistance gene evolution studies, phylogenetic data of the entire SolRgene collection are included, as well as a tool for generating phylogenetic trees of selected groups of germplasm. Data from resistance gene allele-mining studies are incorporated, which enables detection of R gene homologs in related germplasm. Using these resources, various resistance genes have been detected and some of these have been cloned, whereas others are in the cloning pipeline. All this information is stored in the online SolRgene database, which allows users to query resistance data, sequences, passport data of the accessions, and phylogenic classifications.

Conclusion

Solanum section Petota forms the basis of the SolRgene database, which contains a collection of resistance data of an unprecedented size and precision. Complemented with R gene sequence data and phylogenetic tools, SolRgene can be considered the primary resource for information on R genes from potato and wild tuber-bearing relatives.