Schematic representation of the constructs utilized. The AtMHX promoter elements utilized were the repetitive element (RE), unique sequence (US), and minimal promoter (MP) (see Results and Methods for details about these elements). The solid line represents the 5'UTR of AtMHX, the diagonal lines represent the leader intron (LI) of AtMHX, and the dashed line represents the region eliminated from the LI. GUS - the coding sequence of β-glucuronidase; AtMHX ter - the terminator of the AtMHX gene; 35S - the CaMV 35S promoter; NOS ter - the terminator of the Agrobacterium tumefaciens nopaline synthase gene; X - an abolished splice site. The objective of each construct set is explained in the text.
Akua et al. BMC Plant Biology 2010 10:93 doi:10.1186/1471-2229-10-93