Quantitative real-time PCR (qRT-PCR) analyses of 12 selected genes. Leaf tissues were sampled for both inoculated and mock-inoculated plants at 12, 18, 24, 48, 72 and 120 hpi, as well as mock-inoculated near 0 hpi. Three independent biological replications were performed. Relative gene quantification was calculated by comparative ΔΔCT method. All data were normalized to the 18S rRNA expression level. The mean expression value was calculated for every transcript derived fragment (TDF) with three replications.
Wang et al. BMC Plant Biology 2010 10:9 doi:10.1186/1471-2229-10-9