Research article
A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica
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* Corresponding author: Ken Shirasu ken.shirasu@psc.riken.jp
1 Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
2 Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
3 Biotechnology Laboratory, Agricultural Research Corporation, Wad Medani 126, Sudan
BMC Plant Biology 2010, 10:55 doi:10.1186/1471-2229-10-55
Published: 30 March 2010Additional files
Additional file 1:
Distribution of unigene lengths and EST numbers per unigene. (A) Distribution of unigene lengths in the entire S. hermonthica unigene dataset. (B) Distribution of EST numbers per unigene.
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Additional file 2:
Lists of S. hermonthica unigenes that are potentially specific to parasitic plants. Sheet 1- The list of S. hermonthica unigenes that have homologs in T. pusilla or T. versicolor but not in other species databases. Sheet 2- The list of S. hermonthica unigenes that do not have homologs in other known sequences.
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Additional file 3:
Distribution of SSR patterns detected in S. hermonthica ESTs.
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Additional file 4:
Distribution of SSR motifs detected in S. hermonthica ESTs.
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Additional file 5:
SSR information. Sheet1- The list of SSRs analyzed in this study, with SSR ID, primer sequences, and PCR results. The yellow colored linesindicate the markers used in this study.
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Additional file 6:
Examples of PCR results from the amplification of SSR-containing regions in S. hermonthica. (A) Agarose gel images of PCR results using the indicated primer sets and pooled genomic DNAs from the populations listed in Fig. 5. The population numbers correspond to the numbers in Fig. 5A. (B) An agarose gel image showing PCR results using the SSR8 primer set and genomic DNAs extracted from individual plantsfrom the population in Kenya.
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