Table 3 |
|||
|
PCR amplification conditions |
|||
|
Primer pair (upper primer lower primer) |
purpose |
Annealing temperature |
Extension time |
|
|
|||
|
F24D7-9radR F24D7-9radF |
Screening of BAC library |
55.5°C |
1 min |
|
F24D7-13radR F24D7-13radF |
Screening of BAC library |
54°C |
1 min |
|
PPRA:20505U22 PPRA:20954L21 |
RT-PCR on PPR-A and PPR-2 |
52°C |
30 sec |
|
Rfocons1047U22 PPRB:13225L22 |
RT-PCR on PPR-B |
52°C |
1 min 15 sec |
|
PPR1:21229U22 PPRB:13225L22 |
RT-PCR on PPR-1 |
51°C |
1 min |
|
|
|||
|
Hernandez Mora et al. BMC Plant Biology 2010 10:35 doi:10.1186/1471-2229-10-35 |
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