RNA levels of ABI3, ABI5, DREB2A and STZ in wrky18, 40, 60 mutants and wild type seedlings. Seedlings of wild type or mutants were grown on MS medium for 14 days before being transplanted onto MS plates with or without 2.0 μM ABA. RNA was extracted from seedlings on MS medium 12 hours after transplantation. Relative RNA levels of the 4 genes ABI3, ABI5, DREB2A and STZ were analyzed using gene-specific primers by real-time PCR. The means and standard errors were calculated from three independent experiments, all of which included no less than 20 seedlings per sample. Asterisks mark statistically significant differences of expression level between genotypically identical seedlings with or without ABA treatment, by Student-Newman-Keuls Test(p-value < 0.05).
Chen et al. BMC Plant Biology 2010 10:281 doi:10.1186/1471-2229-10-281