Seed oleic acid phenotype and FAD2-1 genotype association analysis for F3 seeds of population 4. Average oleic acid content of soybean seeds produced in Columbia, MO from the segregating F2 population developed from the cross 17D × S08-14788, population 4. X-axis labels indicate a typical commodity soybean line (Williams 82, W82), the two RIL parents, and the RILs grouped by FAD2-1A and FAD2-1B genotype. AA = wild-type FAD2-1A alleles, aa = mutant S117N FAD2-1A alleles derived from 17D, BB = wild-type FAD2-1B alleles, bb = mutant P137R FAD2-1B alleles derived from PI 283327; brackets surround the number of soybean seed samples represented in the genotype class. Error bars indicate plus and minus one standard deviation from the mean. Oleic acid phenotype data of each genotype class is the mean of oleic acid content as a percentage of the total fatty acid content of the oil of all lines carrying the FAD2-1 genotype, five individual samples for each line.
Pham et al. BMC Plant Biology 2010 10:195 doi:10.1186/1471-2229-10-195