Figure 4.

Northern blot analysis of clones belonging to RindPdigS in C. sinensis Navelate fruit's peel after ethylene or 1-MCP pre-treatment. Tissue samples were analysed before any treatment (C0) or after 16 h of treatment with air (A), 500 ppb of 1-MCP (M), or 10 ppm of ethylene (E). In brackets it is indicated whether the clone is a singleton or the cluster it belongs to. Membranes were hybridized with probes corresponding to beta-carotene hydroxylase (N03B01), 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (N03H08), embryo-abundant protein (N04D11), nectarin 5 (N04D12), nectarin 5 (N09B03), 3-phosphoshikimate 1-carboxyvinyltransferase (N10D01), Caffeine synthase (N12F02), GcpE (N13A02), cellular apoptosis susceptibility protein (N13E10), aquaporin (N13F07), cystinosin (N13G07) and acetyltranferase-like protein (N17B07). Hybridization with the C. sinensis 26 S rDNA is shown in the bottom panel. Normalization of hybridization signals was carried out with respect to the hybridization signal of the C. sinensis rDNA. Values below the panels show the relative quantification of the corresponding hybridization signal referred to the value of the sample E. Those hybridization signals lower than two fold the background intensity were not assessed (-).