Open Access Research article

A transcriptomic approach highlights induction of secondary metabolism in citrus fruit in response to Penicillium digitatum infection

Luis González-Candelas1*, Santiago Alamar1, Paloma Sánchez-Torres12, Lorenzo Zacarías1 and Jose F Marcos1

Author Affiliations

1 Departamento de Ciencia de los Alimentos, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Apartado de Correos 73, Burjassot, E46100-Valencia, Spain

2 Insituto Valenciano de Investigaciones Agrarias, Carretera Moncada - Náquera, Km. 4,5. Moncada, E46113-Valencia, Spain

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BMC Plant Biology 2010, 10:194  doi:10.1186/1471-2229-10-194

Published: 31 August 2010

Additional files

Additional file 1:

Macroarray hybridization results from 'Navelina' oranges treated with air (A) or with 10 ppm of ethylene (E) for 16 h, or 24 h after wounding (W) or inoculation with P. digitatum (I) .

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Additional file 2:

Sequence annotation of RindPdigS clones.

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Additional file 3:

Northern blots analysis of RindPdigS genes in control, wounded or P. digitatum infected 'Navelina' fruits at different time points after inoculation. In brackets it is indicated whether the clone is a singleton or the cluster it belongs to Hybridization with the C. sinensis 26 S rDNA is shown in the bottom panel. Normalization of hybridization signals was carried out with respect to the hybridization signal of the C. sinensis rDNA. Values below the panels show the relative quantification of the corresponding hybridization signal referred to the value of the infected sample at 24 hpi. Those hybridization signals lower than two folds the intensity of background were not assessed (-).

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Additional file 4:

Northern blot analysis of clones belonging to RindPdigS in C. sinensis Navelate fruit's peel after ethylene or 1-MCP pretreatment. Tissue samples were analysed before any treatment (C0) or after 16 h of treatment with air (A), 500 ppb xof 1-MCP (M), or 10 ppm of ethylene (E). In brackets it is indicated whether the clone is a singleton or the cluster it belongs to. Hybridization with the C. sinensis 26 S rDNA is shown in the bottom panel. Normalization of hybridization signals was carried out with respect to the hybridization signal of the C. sinensis rDNA. Values below the panels show the relative quantification of the corresponding hybridization signal referred to the value of the sample E. Those hybridization signals lower than two fold the background intensity were not assessed (-).

Format: PDF Size: 180KB Download file

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