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This article is part of the supplement: 4th International Conference of cGMP Generators, Effectors and Therapeutic Implications

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From biochemical and structural studies of soluble guanylate cyclase toward drug design

Emmanuelle Laffly*, Jane Macdonald and Elsa Garcin

Author Affiliations

Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore Maryland, USA

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BMC Pharmacology 2009, 9(Suppl 1):P39  doi:10.1186/1471-2210-9-S1-P39

The electronic version of this article is the complete one and can be found online at:

Published:11 August 2009

© 2009 Laffly et al; licensee BioMed Central Ltd.

Poster presentation

The heterodimeric haemoprotein soluble guanylate cyclase (sGC) is the direct sensor and mediator of nitric oxide (NO) signal transduction via the NO-sGC-cGMP pathway. Aberrant sGC-dependent signalling may be fundamental to the aetiology of a wide variety of cardiovascular pathologies. As a consequence, compounds that activate cGMP production by sGC have a considerable therapeutic potential.

To date, x-ray structures of independent sGC domains HNOX, HNOX-A and GC have been solved. Nevertheless, the determination of the full-length sGC x-ray structure would provide additional clues to understand the structural basis for the mechanism of sGC assembly and regulation and should facilitate the design of these therapeutic agents.

To achieve this goal, I developed a heterologous expression system of full-length bovine sGC (Fl-sGC). Early attempts to produce recombinant bovine sGC in E. coli resulted in misfolded protein accumulation. Indeed, producing soluble protein in Escherichia coli is still a major difficulty in the sGC field. By using fusion technology, I successfully overexpressed both α and β subunits in a soluble heme-bound active form. Optimization of expression levels by varying bacterial growth conditions including temperature, media, additives and induction, will be followed by purification and characterization of Fl-sGC. So, a crucial step has been achieved, allowing us to pursue structural studies to probe the structure and mechanism of sGC and promote the discovery of stimulators of this physiologically important enzyme.