Table 1

Pharmacological characteristics of adrenoceptors in SH-SH5Y and SHβ2AR4 cells.

SH-SY5Y
 SHβ2AR4
Agonist:
 log(Ki)
 log(EC50)
 log(EC50)
EPI
 -7.38 ± .04
 -8.83 ± .06
 -8.22 ± 0.21
UK 14,304
 -7.38 ± .12
 -7.22 ± .36
 -7.72 ± 0.77
Oxymetazoline (OXY)
 -8.85a
 -8.35 ± .47
 N.D.
Isoproterenol
 N.A.
 N.A.
 -7.02 ± 0.28
Antagonist:
 log(Ki)
 Ki Ratio with OXY
Rauwolscine
 -8.82 ± .15
 1.07
 N.A
Yohimbine
 -8.56 ± .17
 1.95
 N.A
Prasozin
 -6.98a
 74.4
 N.A

Binding inhibition and cAMP accumulation studies were performed as described in Methods. The values of the apparent affinity constants Log(Ki) for each competitor were derived from their IC50 values (n = 3–9) using the equation of Cheng and Prusoff [40]. The Log(EC50) values (concentration of the drug that produces 50% of the maximal inhibitory/stimulatory effect of that drug) were calculated by nonlinear regression analysis of the agonist concentration-response curves (n = 3–9) of each agonist. aValues from Kazmi and Mishra [24]. N.D., not determined; N.A., not applicable.

Bawa-Khalfe et al. BMC Pharmacology 2007 7:16   doi:10.1186/1471-2210-7-16