Figure 2.

RT-PCR Products obtained from SH-SY5Y RNA using α₂-AR subtype selective primers. RT-PCR experiments were performed as described in "Methods" using primer pairs recognizing α₂C10/C4 (corresponding to α_2A and α_2C) and α₂C4 (corresponding to α_2C) gene products (Table 3). The reactions amplified fragments of the expected size from each set of primers. α₂C10/C4 primers amplified 233 bp products from SH-SY5Y mRNA that were sensitive to digestion by BglII (specific for the α_2A product). Restriction digestion with BstXI of the 630 bp product of α 2C4 primer amplification gave three fragments of 271, 225 and 78 bp. All reactions were performed in the presence (+) or absence (-) of reverse transcriptase (RT) to rule out the possibility of DNA contamination. Lane M designates the 100 bp ladder; the 500 bp fragment is indicated by an arrow in each panel.