Effect of SB220025 on JNK activation. Cells were stimulated with LPS and SB220025. Incubations were terminated after 1 h and parallel immunoblots were run from same cell lysates using antibodies against Thr183/Tyr185 phosphorylated JNK (pp54 and pp46), total JNK (p54 and p46). Phosphorylated JNK values were normalised to total JNK values. The result is representative of three experiments with similar results.
Lahti et al. BMC Pharmacology 2006 6:5 doi:10.1186/1471-2210-6-5