Figure 5.

Effects of actinomycin D on TPA-induced 6-Keto-PGF_1α production. Cells were preincubated with 1.0 μM actinomycin D for 2-h and then incubated in the presence and absence of 0.6 μM epoxomicin and/or 16.7 μM TPA for another 6-h. The analyses were performed with triplicate dishes. *- Statistically significant vs TPA in the absence of actinomycin D. **- Statistically significant vs epoxomicin plus TPA in the absence of actinomycin D.