Table 4

Transgene expression in different human cancer cell lines. These values are determined in the same experiments as depicted in figures 7 and 8. A: Infectibility of the cell lines as defined by the percentage of adenovirus DNA seeded that was detected in the cells 2 days after infection. B: the transcription efficiency is defined by the ratio of the mRNA values divided by the number of adenoviral copies in the cells. C: The translation efficiency is the ratio of the luciferase activity / luciferase mRNA. D: Luciferase activity in 103 RLU / mg protein. The absolute values are shown in italic. The values relative to the LS174T, which was arbitrary set as reference are indicated in plain text.
A









Infectibility (% Ad in cell / Ad seeded)
MOI
 Cell lines

U251
 NCI H460
 HepG2
 A549
 LS 174T
1
 72
 82
 13
 15
 12
 14
 34
 38
 0.9
 1
10
 55
 104
 7
 13
 10
 18
 21
 40
 0.5
 1
100
 78
 101
 10
 13
 9
 12
 22
 29
 0.8
 1
Average
96
14
15
36
1
SD
12
1
3
6

B









Transcription efficiency (mRNA / Ad DNA
MOI
 Cell lines

U251
 NCI H460
 HepG2
 A549
 LS 174T
1
 2.1
 0.7
 2.1
 0.8
 2.0
 0.7
 0.4
 0.14
 2.8
 1
10
 2.0
 0.7
 2.7
 0.9
 1.9
 0.7
 0.4
 0.16
 2.8
 1
100
 1.9
 0.7
 2.7
 1.0
 2.3
 0.9
 0.6
 0.22
 2.6
 1
Average
0.72
0.91
0.76
0.17
1
SD
0.02
0.14
0.11
0.04

C









Translation efficiency (Luciferase / mRNA)
MOI
 Cell lines

U251
 NCI H460
 HepG2
 A549
 LS 174T
1
 40
 2.8
 12
 0.8
 13
 0.9
 9
 0.6
 14
 1
10
 127
 2.2
 48
 0.8
 38
 0.7
 28
 0.5
 58
 1
100
 316
 1.6
 152
 0.8
 140
 0.7
 78
 0.4
 198
 1
Average
2.2
0.8
0.8
0.5
1
SD
0.6
0.04
0.2
0.1

D









Luciferase activity
MOI
 Cell lines

U251
 NCI H460
 HepG2
 A549
 LS 174T
1
 60
 172
 3
 9.2
 3
 9.5
 1
 3.4
 0.3
 1
10
 1381
 158
 92
 10.5
 69
 7.9
 27
 3.1
 9
 1
100
 46350
 116
 4061
 10.1
 3055
 7.6
 1016
 2.5
 400
 1
Average
149
9.9
8.3
3.0
1
SD
29
0.7
1.0
0.4

Lefesvre et al. BMC Pharmacology 2003 3:11   doi:10.1186/1471-2210-3-11