Figure 5.

RNase protection assay demonstrating the distribution of EP₁, EP₂, EP₃, EP₄ and GAPDH mRNA in rabbit vascular tissues. The protected fragments were electrophoretically separated on a 6% agarose/7 M urea gel. The film was exposed for 36 hours. A 100 bp ladder was used as a size marker. Panel A: EP₁, EP₂, EP₃, and EP₄ expression in rabbit aorta. Panel B: EP₂ and EP₄ expression in preglomerular microvessels in the kidney. Panel C: EP₂ expression in the rabbit ear artery, jugular vein and vena cava.