Effect of CCl4 with or without prior administration of natansnin on PARP cleavage. Whole cell protein extracted from control, CCl4 and natansnin (10 mg/kg, 20 mg/kg body weight) treated rats and separated on 12% SDS gels and transferred on to nitrocellulose membranes. Immunoblot was detected with PARP specific primary antibody. The expression levels of β-actin were used as loading controls. Densitometric analysis showing the protein levels of PARP were increased in CCl4 group while treatment with natansnin significantly decreased the protein levels. Data are represented as mean ± standard deviation from three independent experiments (*p < 0.05 indicate significant difference relative to the CCl4 treated rats).
Srilaxmi et al. BMC Pharmacology 2010 10:13 doi:10.1186/1471-2210-10-13