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Stimulation of purified sGC in the presence and absence of DTT by the NO donor SNP. YC-1 and the ortho-. meta- and para-PALs with Mg2+ as cofactor. Shown are the x-fold stimulations versus basal specific activity of SNP (1 μM). YC-1 (100 μM, 10 μM, 1 μM and 1 μM YC-1 in combination with 1μM SNP) and the different PALs (100 μM, 10 μM, 1 μM and 1 μM PAL in combination with 1 μM SNP). The data presented represent the mean ± SEM out of four independent experiments performed in duplicate. |
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| Stimulation of purified sGC [fold increase vs. basal activity ± SEM] (basal specific activity [nmol/mg/min]) |
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| Stimulator |
Concentration |
+ DTT |
-DTT |
|
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| Basal |
1.0 ± 0.1 (64 ± 8) |
1.0 ± 0.2 (150.8 ± 33.4) |
|
| SNP |
1 μM |
82.3 ± 10.5 |
17.1 ± 2.2 |
| 100 μM |
45.9 ± 7.7 |
44.4 ± 4.8 |
|
| YC-1 |
10 μM |
4.5 ± 0.8 |
7.0 ± 1.7 |
| 1 μ M |
2.0 ± 0.4 |
1.5 ± 0.2 |
|
| YC-1 + SNP |
1 μM + 1 μM |
98.4 ± 17.3 |
20.6 ± 3.6 |
| 100 μM |
14.4 ± 1.6 |
21.4 ± 4.2 |
|
| ortho-PAL |
10 μM |
2.9 ± 1.5 |
3.1 ± 0.6 |
| 1 μ M |
2.1 ± 1.0 |
1.0 ± 0.1 |
|
| ortho-PAL + SNP |
1 μM + 1 μM |
85.7 ± 11.4 |
15.8 ± 2.0 |
| 100 μM |
54.7 ± 10.2 |
56.1 ± 0.1 |
|
| meta-PAL |
10 μM |
21.3 ± 7.1 |
23.3 ± 4.1 |
| 1 μ M |
4.5 ± 1.0 |
4.9 ± 0.4 |
|
| meta-PAL + SNP |
1 μM + 1 μM |
185.6 ± 72.6 |
39.0 ± 5.6 |
| 100 μM |
36.6 ± 3.5 |
30.2 ± 4.5 |
|
| para-PAL |
10 μM |
15.3 ± 2.7 |
17.3 ± 1.9 |
| 1 μM |
3.5 ± 0.4 |
3.8 ± 0.3 |
|
| para-PAL + SNP |
1 μM + 1 μM |
229.1 ± 33.6 |
50.6 ±5.5 |
Becker et al. BMC Pharmacology 2001 1:13 doi:10.1186/1471-2210-1-13 |
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