Figure 5.

APα-induced [Ca2+]i rises are mediated by the GABAA receptor. (A) Picrotoxin completely abolished APα-induced [Ca2+]i rises. Fluorescent Fura2-AM imaging of 3 DIV hippocampal neurons incubated with or without 100 mM picrotoxin (a non-competitive GABAA receptor antagonist) 30 minutes prior to and continuously throughout imaging. APα (500 nM) or vehicle was added at the time-point indicated by the arrow. The graph is representative of the four experiments conducted (N = 36 neurons/condition/experiment). (B) Bicuculline completely abolished APα-induced [Ca2+]i rises. Fluorescent Fura2-AM imaging of 3 DIV hippocampal neurons incubated with or without 30 μM bicuculline (a competitive GABAA receptor antagonist) for 30 minutes prior to and continuously throughout imaging. APα (500 nM) or vehicle was added at the time-point indicated by the arrow. The graph is representative of the four different experiments conducted (N = 36 neurons/condition/experiment).

Wang and Brinton BMC Neuroscience 2008 9(Suppl 2):S11   doi:10.1186/1471-2202-9-S2-S11