APα-induced [Ca2+]i rise shows dose- and DIV-dependent manner and is stereoisomer-specific. (A) Bar graph shows dose responses of APα on [Ca2+]i rise in 3 DIV neurons. Data are mean ± SEM (N = 3–6 experiments with 36 neurons/condition/experiment, *p < 0.05, **p < 0.01). (B) Stereospecificity of APα-induced [Ca2+]i. Neurons were exposed to 250 nM of the stereoisomers APα, 3α5βAP, 3β5αAP, 3β5βAP or progesterone (250 nM) at the time-point indicated by the arrow. The graph is a representative of four different experiments (N = 36 neurons/condition/experiment). (C) Bar graph shows the summary of data collected from stereoisomer experiments and represented as [Ca2+]i. **p < 0.01 APα or 3α5βAP compared to control, or APα and 3α5βAP compared to each other; N = 4 experiments with 36 neurons/condition/experiment. (D) Bar graph indicates the percentage of neurons that exhibit a [Ca2+]i rise in response to 500 nM APα at different days in vitro (DIV). DIV 0 is defined as the day the neurons are seeded. The greatest response, around 90% of cells responding to APα, is observed on 3 DIV. Data are mean ± SEM (N = 3 independent experiments with at least 36 neurons/condition/experiment). *p < 0.01, **p < 0.001 versus DIV 0.
Wang and Brinton BMC Neuroscience 2008 9(Suppl 2):S11 doi:10.1186/1471-2202-9-S2-S11