A large population of diverse neurons in the Drosophila central nervous system expresses short neuropeptide F, suggesting multiple distributed peptide functions
1 Department of Zoology, Stockholm University, S-10691 Stockholm, Sweden
2 Emmy Noether Neuropeptide Group, Department of Biology, Animal Physiology, Philipps-University, D-35032 Marburg, Germany
BMC Neuroscience 2008, 9:90 doi:10.1186/1471-2202-9-90Published: 19 September 2008
Rotating view of sNPFp-IR neurons in the ventral nerve cord of a third instar larva.
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Rotating view of sNPFp-IR neurons in the brain of a third instar larva. The most prominent labeling is seen in the mushroom body lobes. For further details see Fig. 2D.
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Additional file 3:
Direct MALDI-TOF profiling of protocerebrum fragments containing the mushroom bodies.A – C Direct peptide profiling in larva (A) and adult (B – C) brain tissue. Typically, the most intense mass peak corresponded to the theoretical mass of sNPF-14–11 or sequence-identical sNPF-212–19 with the sequence SPSLRLRFa (labeled sNPF, theoretical monoisotopic mass 974.60 Da). In one out of ten preparations, a small mass peak corresponding to the full length sNPF-1 was found (B). Other predicted sNPF-peptides could not be detected. D MALDI-TOF-TOF fragmentation of the mass peak at 974.6 from adult tissue. The measured b and y fragments clearly indicate that this mass peak corresponds to SPSLRLRFa, although the parent material was too small to obtain a full fragment ion series.
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