Furosemide selectively depressed GABAA fast spontaneous events. Population (n = 5 cells) summary for spontaneous IPSCs in control and furosemide treated conditions. A) Representative recording or spontaneous events in control and furosemide treated conditions. B) The average amplitude of slow sIPSCs was not significantly different between control and furosemide treated conditions (mean = 43 ± 12 pA and mean = 40 ± 9 pA respectively). C) The amplitude of sIPSCs was significantly reduced (mean control = 45 pA, mean treated = 16 pA) with treatment of furosemide (** indicates p < 0.001, Wilcoxon signed-rank test). D-E) The frequency of spontaneous IPSCs in the presence of furosemide was not significantly reduced for fast (mean = 2.2 ± 3.7 Hz, mean = 1.4 ± 2.6 Hz, control and treated respectively) or slow events (mean = 0.012 ± 0.004 Hz, mean = 0.019 ± 0.0034 Hz, control and treated respectively).
Sceniak and MacIver BMC Neuroscience 2008 9:8 doi:10.1186/1471-2202-9-8