Additional file 2.
Comparison of the effect different laminin concentrations on mouse neurosphere numbers. Mouse neurospheres were dissociated and plated at 500 cells/well in medium containing 5–20 μg/ml laminin. After 7 days, the number of neurospheres formed was quantified. Compared to wells with no added laminin, significant increases were observed for all concentrations (p < 0.01). However, whilst 10 μg/ml laminin produced significantly more neurospheres than 5 μg/ml (p < 0.01), no significant difference was found between 10 or 20 μg/ml laminin. Therefore, 10 μg/ml laminin was used for all experiments on human cells. Statistics were determined by a one-way ANOVA with Tukey post-test, with 8 technical replicates performed within a single experiment.
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Hall et al. BMC Neuroscience 2008 9:71 doi:10.1186/1471-2202-9-71