A circadian rhythm in Period2 mRNA in the hippocampus of VIP-deficient mice as measured by ISH. A) Film images of ISH for Period2 on tissue sections taken from WT and VIP-deficient mice sacrificed during the subjective day (CT10; a, b) and subjective night (CT 23; c, d). The scale bar equals 0.5 mm. B) Optical density measurements of Period2 labeling in the hippocampus of WT and VIP-deficient mice during the subjective day (CT 10, white bars) and subjective night (CT 23, black bars). The Period2 hybridization signal was significantly (P < 0.05) higher in the subjective night in the hippocampus of both lines of mice.
Chaudhury et al. BMC Neuroscience 2008 9:63 doi:10.1186/1471-2202-9-63