Tmem10 is a type I transmembrane protein. (A) Alignment of mouse, rat and human Tmem10 amino acid sequence. Black/grey box indicates the predicted position of the transmembrane domain. (B) Oli-neu cells were transfected to express either N-terminal ECFP-tagged or C-terminal EYFP-tagged Tmem10. Subsequent staining with anti-GFP antibody was performed either on living, unpermeabilized (surface anti-GFP) or on fixed, permeabilized (after permeabilization anti-GFP) cells. Scale bars, 10 μm.
Kippert et al. BMC Neuroscience 2008 9:40 doi:10.1186/1471-2202-9-40