Table 1

The numbers of β-gal or PkcΔGG positive cells that contained TH-IR, in rats sacrificed at 4 days after co-injection of pTHlac/gC – bdnf and pHSVpkcΔGG/gC – bdnf into the midbrain


Numbers of positive cells
%
Numbers of positive cells
%
Rat
β-gal-IR
TH-IR
Costained
Flag-IR
TH-IR
Costained

1
347
287
83
328
232
71
2
322
278
86
205
134
66
3
340
274
81
470
354
75
4
418
358
86
310
212
68
Average


84 ± 1


70 ± 2

A mixture of equal IVP/ml of pTHlac/gC – bdnf and pHSVpkcΔGG/gC – bdnf (for titers and injection conditions, see methods) was injected into the midbrain proximal to the substantia nigra. The rats were perfused at 4 days after gene transfer, the brains were sectioned, and alternating sections were costained for either β-gal-IR or flag-IR and TH-IR. Sufficient sections were examined to count at least 200 cells that contained either β-gal-IR or flag-IR in each rat. In the sections that were examined, all of the β-gal-IR or flag-IR cells were scored for containing or lacking TH-IR. For the average % costaining, the means ± sems are shown.

Cao et al. BMC Neuroscience 2008 9:37   doi:10.1186/1471-2202-9-37