|
The numbers of β-gal or PkcΔGG positive cells that contained TH-IR, in rats sacrificed at 4 days after co-injection of pTHlac/gC – bdnf and pHSVpkcΔGG/gC – bdnf into the midbrain |
||||||
| Numbers of positive cells |
% |
Numbers of positive cells |
% |
|||
| Rat |
β-gal-IR |
TH-IR |
Costained |
Flag-IR |
TH-IR |
Costained |
|
|
||||||
| 1 |
347 |
287 |
83 |
328 |
232 |
71 |
| 2 |
322 |
278 |
86 |
205 |
134 |
66 |
| 3 |
340 |
274 |
81 |
470 |
354 |
75 |
| 4 |
418 |
358 |
86 |
310 |
212 |
68 |
| Average |
84 ± 1 |
70 ± 2 |
||||
|
A mixture of equal IVP/ml of pTHlac/gC – bdnf and pHSVpkcΔGG/gC – bdnf (for titers and injection conditions, see methods) was injected into the midbrain proximal to the substantia nigra. The rats were perfused at 4 days after gene transfer, the brains were sectioned, and alternating sections were costained for either β-gal-IR or flag-IR and TH-IR. Sufficient sections were examined to count at least 200 cells that contained either β-gal-IR or flag-IR in each rat. In the sections that were examined, all of the β-gal-IR or flag-IR cells were scored for containing or lacking TH-IR. For the average % costaining, the means ± sems are shown. | ||||||
Cao et al. BMC Neuroscience 2008 9:37 doi:10.1186/1471-2202-9-37 |
||||||