Table 1

List of proteins identified in synaptosomes and crude cytosol from hippocampal culture or tissue
Spot number	Accession number*	Protein name	S tis	S cul	C cul	Method of detection

Cytoskeletal and their interacting Proteins(CK)
4	P60711	Actin, cytoplasmic	x	x	x	2DE-MS
37	P63259	Actin, cytoplasmic 2	x	x	x	2DE-MS
48	Q61274	Alpha-cardiac actin [Fragment]	x	x		2DE-MS
100, 101, 123	P68370	Tubulin alpha-1 chain	x	x	x	2DE-MS
36, 39, 47,124	P04691	Tubulin beta chain	x	x	x	2DE-MS
22	P37805	Neuronal protein 25	x	x		2DE-MS
53	P13668	Stathmin	x	x	x	2DE-MS
119	P13668	Stathmin			x	2DE-MS
55	Q63228	Glia maturation factor beta	x	x	x	2DE-MS
33	P45592	Cofilin, non-muscle isoform	x	x	x	2DE-MS
92	Q9R0P5	Destrin	x	x	x	2DE-MS
50	P63029	Translationally controlled tumor protein	x			2DE-MS
125	P62963	Profilin I		x	x	2DE-MS
127	Q9EPC6	Profilin II		x	x	2DE-MS
Metabolism(M)
18, 105, 106	P04797	Glyceraldehyde-3-phosphate dehydrogenase	x	x	x	2DE-MS
71	P16617	Phosphoglycerate kinase, testis specific	x	x	x	2DE-MS
96	P10719	ATP synthase beta chain, mitochondrial	x	x	x	2DE-MS
11	P31399	ATP synthase D chain, mitochondrial	x	x		2DE-MS
26, 27	P04764	Alpha enolase	x	x	x	2DE-MS
84	P11980	Pyruvate kinase, M2 isozyme	x	x	x	2DE-MS
78	Q9ER34	Mitochondrial aconitase [Precursor]	x	x	x	2DE-MS
2, 67	P10860	Glutamate dehydrogenase, mitochondrial [Precursor]	x	x	x	2DE-MS
34, 35, 64	P04636	Malate dehydrogenase, mitochondrial [Precursor]	x	x		2DE-MS
49	P11240	Cytochrome c oxidase polypeptide VA	x	x	x	2DE-MS
8, 85	Q99NA5	NAD+specific isocitrate deihydrogenase a-subunit	x	x		2DE-MS
83	P42123	L-lactate dehydrogenase B chain	x	x	x	2DE-MS
5	P48500	Triosephosphate isomerase	x	x	x	2DE-MS
31, 103	P05065	Fructose-biphosphate Aldolase A	x	x	x	2DE-MS
40	P09117	Fructose-bisphosphate aldolase C	x	x	x	2DE-MS
41, 68	Q9D0K2	Succinil-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial	x	x	x	2DE-MS
56	Q05982	Nucleoside diphosphate kinase A	x	x	x	2DE-MS
61	P19804	Nucleoside diphosphate kinase B	x	x	x	2DE-MS
69	P09606	Glutamine synthetase	x	x	x	2DE-MS
70	P25809	Creatine kinase, ubiquitous mitochondrial [Precursor]	x		x	2DE-MS
75	P13221	Aspartate aminotransferase cytoplasmatic	x	x	x	2DE-MS
122	P07335	Creatine kinase, B chain	x	x	x	2DE-MS
1, 82	O88989	Cytosolic malate dehydrogenase	x	x	x	2DE-MS
80	P97532	3-mercaptopyruvate sulfurtransferase	x			2DE-MS
90	P04764	Alpha-enolase	x		x	2DE-MS
104	Q80YG1	Phosphoserine aminotransferase		x	x	2DE-MS
113, 114	P48500	Triosephosphate isomerase		x	x	2DE-MS
16	P19234	NADH-ubiquinone oxidoreductase 24 kDa subunit	x	x	x	2DE-MS
Synaptic components (SY)
72, 76, 86, 87	P47942	Dihydropyrimidinase related protein-2 (drp-2)	x	x	x	2DE-MS
89	Q62952	Dihydropyrimidinase related protein-3 (drp-3)	x	x	x	2DE-MS
3	Q63537	Synapsin II	x	x	x	2DE-MS
29	P07825	Synaptophysin	x	x		WB
30	P32851	Syntaxin 1 A-E	x	x		WB
46	P37377	Alpha-synuclein	x	x		2DE-MS
45	Q63754	Beta-synuclein	x	x		2DE-MS
73	P25286	Vacuolar proton translocating ATPase subunit a isoform 1	x	x		2DE-MS
9, 51	P31044	Phosphatidylethanolamine-binding protein 1	x	x	x	2DE-MS
19	P55051	Fatty acid-binding protein, brain	x	x	x	2DE-MS
126	P11030	Acyl-CoA-binding protein		x	x	2DE-MS
128	P62775	Myotrophin			x	2DE-MS
Receptors, Ion Channel and adhesion molecules (I)
23, 63	Q6P9W9	Voltage-dependent anion-selective channel protein 1	x	x	x	2DE-MS
66	P50516	Vacuolar ATPsynthase catalytic subA	x	x		2DE-MS
Vesicle Transport and Recycling (T)
97	P46462	Transitional endoplasmic reticulum ATPase	x		x	2DE-MS
25	P63012	Ras-related protein rab-10	x	x	x	WB
28	Q62824	Exocyst complex component Sec8		x		WB
Protein Fate (synthesis, folding, modification and destination) (PF)
38	P63039	60 kda heat shock protein, mitochondrial precursor (hsp60)	x	x	x	2DE-MS
65	P63017	Heat shock 70 kDa protein-ps1	x	x	x	2DE-MS
102	P07901	Heat shock protein [Fragment]			x	2DE-MS
44	P48721	Stress-70 protein, mitochondrial [Precursor]	x	x	x	2DE-MS
17	Q00981	Ubiquitin carboxyl-terminal hydrolase isozyme L1	x	x		2DE-MS
15	P20108	Mitochondrial thioredoxin-dependent peroxide reductase	x	x	x	2DE-MS
52	Q9DB15	39S ribosomal protein L12, mitochondrial	x	x		2DE-MS
6	P52555	Endoplasmic reticulum protein ERp29	x	x	x	2DE-MS
32, 98, 99	P10111	Peptidyl-prolyl cis-trans isomerase A	x	x	x	2DE-MS
42, 43, 79	P11598	Protein disulfide isomerase A3	x	x	x	2DE-MS
77	O35814	Stress induced phosphoprotein 1	x	x	x	2DE-MS
88	P11983	T complex polypeptide 1b	x	x	x	2DE-MS
21	P55053	Fatty acid-binding protein, epidermal	x	x	x	2DE-MS
59	P07483	Fatty acid-binding protein, heart	x	x	x	2DE-MS
57	Q9EQX9	Ubiquitin-conjugating enzyme e2	x	x	x	2DE-MS
108, 109	P04905	Glutathione S-transferase Yb-1			x	2DE-MS
107	P04904	Glutathione S-transferase Yc-1			x	2DE-MS
112	P08010	Glutathione S-transferase Yb-2			x	2DE-MS
111	P08009	Glutathione S-transferase Yb-3			x	2DE-MS
116	P14942	Glutathione S-transferase alpha-4			x	2DE-MS
110	Q63716	Peroxiredoxin-1		x	x	2DE-MS
54	P35704	Peroxiredoxin-2	x	x	x	2DE-MS
93	Q9R063	Peroxiredoxin-5, mitochondrial [Precursor]	x	x	x	2DE-MS
94	Q63347	26S protease regulatory subunit 7			x	2DE-MS
115	P17220	Proteasome subunit alpha type 2		x	x	2DE-MS
91	Q9JHW0	Proteasome subunit beta type 7 [Precursor]	x		x	2DE-MS
Signalling Proteins (S)
24	P10354	Chromogranin A	x	x		WB
74	P50399	RAB GDP dissociation inhibitor beta-2	x	x	x	2DE-MS
10	P60766	Cell division control protein 42 homolog	x	x		2DE-MS
60	P70349	Histidine triad nucleotide-binding protein	x	x	x	2DE-MS
81	P14669	Annexin III	x			2DE-MS
117	Q62658	FK506-binding protein 1°		x	x	2DE-MS
120	P62260	14-3-3 protein epsilon		x	x	2DE-MS
121	P63102	14-3-3 protein zeta/delta		x	x	2DE-MS
62	P62994	Growth factor receptor bound protein 2	x	x		2DE-MS
Other (O)
12	O35264	Platelet-activating factor acetylhydrolase IB subunit beta	x		x	2DE-MS
13	P67779	Prohibitin	x	x	x	2DE-MS
7	O35244	Antioxidant protein 2	x	x	x	2DE-MS
20	P07632	Superoxide dismutase [Cu-Zn]	x	x	x	2DE-MS
58	Q9CPT4	IL25	x	x	x	2DE-MS
14	O88767	CAP1 PROTEIN	x	x	x	2DE-MS
118	P01041	Cystatin B			x	2DE-MS
95	Q8CDL9	Similar to hypothetical protein	x	x	x	2DE-MS

S: synaptosome; C: crude cytosol; cul: culture; tis: tissue; 2DE-MS: two-dimensional electrophoresis and MALDI-TOF mass spectrometry; WB: Western blot; * Swiss-Prot database; Spot numbers correspond to the spot numbers indicated in Fig. 1 and 4.
Corti et al. BMC Neuroscience 2008 9:36 doi:10.1186/1471-2202-9-36