Table 4

Genes differentially expressed 24h or 28d following carrageenan injection and known to be involved in pain.

GenBank

Description

Name

P-value

Cluster

Selection criteria

References


1. Membrane receptors and their ligands


NM_022225

5-hydroxytryptamine (serotonin) receptor 1b

Htr1b

0.0005

A

w

[48]; [49]

NM_012685

vasoactive intestinal peptide receptor 1

Vipr1

0.0017

A

w

[50]

U30290

galanin receptor 1

Galr1

0.0095

B

w

[51]

BI395810

neuropeptide y receptor y1

Npy1r

0.0085

B

w

[52]

NM_017155

adenosine a1 receptor

Adora1

0.0034

E

w

[53]

U28430

5-hydroxytryptamine (serotonin) receptor 3a

Htr3a

0.0002

E

w

[48]

NM_022695

neurotensin receptor 2

Ntsr2

0.0001

E

w

[54]

BF412731

prodynorphin

Pdyn

0.0000

F

w, z

[55]

M11596

calcitonin-related polypeptide, beta

Calcb

0.0000

G

w, z

[56]

BG376217

glutamate receptor, ionotropic, ampa1 (alpha 1)

Gria1

0.0073

H

w

[57]

NM_017011

glutamate receptor, metabotropic 1

Grm1

0.0000

H

w

[37]

NM_012870

neuropeptide y receptor y5

Npy5r

0.0021

H

w, z

[32]; [58]


2. Ion channels and regulator of ion homeostasis


AI010839

potassium large conductance calcium-activated channel, subfamily m, alpha member 1

Kcnma1

0.0050

A

w

[59]

M34052

potassium voltage gated channel, shaw-related subfamily, member 2

Kcnc2

0.0014

B

w

[59]

AI717104

potassium inwardly-rectifying channel subfamily J member 14

Kcnj14

0.0003

B

w

[59]

NM_032073

potassium voltage-gated channel, subfamily q, member 1

Kcnq1

0.0019

B

w

[59]

AI137995

sodium channel, voltage-gated, type iv, beta

Scn4b

0.0087

B

w

[60]

NM_012618

s100 calcium-binding protein a4

S100a4

0.0066

E

w

[61]

NM_053587

s100 calcium binding protein a9 (calgranulin b)

S100a9

0.0002

F

w, z

[62; 62]

X04280

calbindin 1

Calb1

0.0016

G

w, z

[63]


3. cAMP and adenylate cyclase cascade


M12672

guanine nucleotide binding protein, alpha inhibiting 2

Gnai2

0.0028

A

y

[64]

U39572

phosphatidylinositol 4-kinase, catalytic, alpha polypeptide

Pik4ca

0.0096

A

w

[65]

NM_053926

phosphatidylinositol-4-phosphate 5-kinase, type ii, alpha

Pip5k2a

0.0067

A

w

[65]

BF410504

g-protein-coupled receptor gpr34

GPR34

0.0000

D

y

[66]

NM_053456

phospholipase c-like 1

Plcl1

0.0000

D

w

[67]

BM386789

regulator of g-protein signaling 1

Rgs1

0.0071

E

w

[68]

BI281632

regulator of g-protein signalling 10

Rgs10

0.0008

F

w

[68]

NM_017142

adenylyl cyclase 8

Adcy8

0.0098

G

y

[35]

NM_134386

g protein-coupled receptor associated sorting protein 1

GASP1

0.0071

G

w, y

[69]

BF565001

phosphodiesterase 4d

Pde4d

0.0053

G

w

[70]

AY043246

regulator of g-protein signaling 2

Rgs2

0.0003

G

w

[38]

NM_017214

regulator of g-protein signaling 4

Rgs4

0.0074

G

w

[38]

NM_080411

g protein-coupled receptor 83

Gpr83

0.0052

H

y

[66]


4. MAPK cascade


BG664160

mitogen-activated protein kinase kinase kinase kinase 3

Map4k3

0.0027

A

w

[34]

AF092450

mitogen activated protein kinase 8 interacting protein

Mapk8i p

0.0001

A

w

[34]

NM_133545

protein tyrosine phosphatase 2e

Ptpn21

0.0011

A

w

[71]

AI102620

mitogen activated protein kinase kinase kinase 1

Map3k1

0.0027

B

w

[33]

BI290864

mitogen activated protein kinase kinase kinase 3

Map3k3

0.0098

B

w

[33]

AJ271834

protein phosphatase 1b, magnesium dependent, beta isoform

Ppm1b

0.0000

B

w

[72]

BE113127

protein phosphatase 3, catalytic subunit, alpha isoform

Ppp3ca

0.0029

B

w

[72]

AA924620

rab40b, member ras oncogene family

Rab40b

0.0068

B

y

[73]

AI233712

protein phosphatase 1d magnesium-dependent, delta isoform

Ppm1d

0.0011

D

y

[72]

AI104546

protein tyrosine phosphatase 4a3

Ptp4a3

0.0067

E

y

[72]

NM_130403

protein phosphatase 1, regulatory (inhibitor) subunit 14a

Ppp1r1 4a

0.0000

E

y

[72]

AI411122

rab1, member ras oncogene family

Rab1

0.0013

H

y

[73]

BM384841

rab15, member ras onocogene family

Rab15

0.0044

H

y

[73]


5. Growth factors and related proteins


NM_012513

brain derived neurotrophic factor

BDNF

0.0067

B

w, z

[43] [42]

XM_231739

IGF2 binding protein 3

Igf2bp3

0.0091

D

w

[74]

NM_013104

IGF binding protein 6

Igfbp6

0.0020

E

w

[74]

BE102996

neurotrophic tyrosine kinase, receptor, type 2

Ntrk2

0.0042

E

w

[13]

U02315

neuregulin 1

Nrg1

0.0022

G

w, z

[75]


6. Arachidonic acid cascade and mediators of inflammation


NM_138547

3-alpha-hydroxysteroid dehydrogenase

0.0055

C

w

[76]

U03389

prostaglandin-endoperoxide synthase 2

cox2

0.0019

D

w

[77]

J04488

prostaglandin d2 synthase

Ptgds

0.0017

E

w

[78]

NM_017020

interleukin 6 receptor

Il6ra

0.0030

F

w

[76]

AA851740

interleukin 6 signal transducer

Il6st

0.0025

F

w

[76]

NM_133551

phospholipase a2, group 4a

Pla2g4a

0.0024

F

w

[79]

AA893169

tissue inhibitor of metalloproteinase 3

Timp3

0.0060

F

w

[9]


7. Others


NM_012823

annexin a3

Anxa3

0.0026

A

w,z

[14]

BI281979

microtubule-associated proteins 1b

Map1lc 3b

0.0076

A

y, z

[15]

AB011679

tubulin, beta 5

Tubb5

0.0019

A

y

[17]

NM_023974

synaptoporin

Synpr

0.0009

B

y, z

[16]

NM_012551

early growth response 1

Egr1

0.0037

B

w

[29]

BI294912

proteasome subunit, alpha type 3

Psma3

0.0020

D

w

[80]

BI283657

chaperonin subunit 4 (delta)

Cct4

0.0001

F

y

[18]

BG372598

neural cell adhesion molecule 1

Ncam1

0.0045

F

w,z

[81]

NM_012755

fyn proto-oncogene

Fyn

0.0010

E

w

[19]


For cluster description see Result and Figure 3.

Selection criteria:

w – involved in pain-related pathways.

y – interact with or regulate protein involved in pain perception.

z- expression level is altered in different model of pain.

Yukhananov and Kissin BMC Neuroscience 2008 9:32   doi:10.1186/1471-2202-9-32

Open Data