Comparison of microarray and Q-PCR results. Equal amounts of total RNA from each sample have been used for PCR amplification. The results were corrected for difference in PCR efficiency based on the dilution curves for each gene. Each gene expression was normalized to reference genes (sep15 and gtpbp4) to correct for possible error in RNA measurement. Data presented as expression relative to reference genes ± SEM. There were 8 control and 9 28d-group samples. p-value is unpaired t-test. Relative expression is presented as percentage of control.
Yukhananov and Kissin BMC Neuroscience 2008 9:32 doi:10.1186/1471-2202-9-32