Figure 4.

DCX is expressed in GRP neurons and a sub-population of VIP neurons. Photomicrographs of adult rat hypothalamus showing the comparative distribution of DCX and neuropeptide immunoreactivity. A&B. Adjacent sections showing DCX and somatostatin (SSt). C&D. Adjacent sections showing DCX and GRP. E&F. Adjacent sections showing DCX and VIP. Scale bar = 100 μm. G-I. Dual colour immunohistochemistry showing extensive co-localization of DCX and GRP. Co-localizing neurons are indicated by vertical arrows. An individual neuron with low but detectable GRP is indicated with a horizontal arrow. In G, a DCX+/GRP-negative neuron is indicated with a vertical arrow. J-L. Dual colour immunohistochemistry showing partial co-localization of DCX and VIP. Co-localizing neurons are indicated by vertical arrows in L. In J, a DCX+/VIP-negative neuron is indicated, and in K, a VIP+/DCX-negative neuron is indicated. Scale bar = 20 μm

Geoghegan and Carter BMC Neuroscience 2008 9:2   doi:10.1186/1471-2202-9-2
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