Figure 9.

Effects of α-methylserotonin, carbachol, and phenylephrine on CDP-diacylglycerol production, inositol phosphate accumulation and phosphatidylinositide synthesis. Slices of prefrontal cortical or hippocampal tissues were pre-labeled with [³H]cytidine or [³H]inositol in parallel, and then incubated in the presence of 5 mM LiCl. Indicated concentrations of the agonists were added for 90 min, followed by assay of the levels of [³H]CDP- diacylglycerol, [³H]inositol phosphates, and [³H]phosphoinositides. Calculated ratios of CDP- diacylglycerol over inositol phosphates (CDP-DG/IP ratios) are shown in the rightmost panel relative to the scale on the right y-axis. Each bar is the mean ± SEM (n = 15 for α-methylserotonin, 12 for carbachol, 9 for phenylephrine). Data were separately analyzed by One-way ANOVA for each receptor agonist. *p < 0.01, Dunnett test compared to the respective control (zero drug concentration).