Effects of SKF38393 on CDP-diacylglycerol production, inositol phosphate accumulation and phosphatidylinositide synthesis. Prefrontal cortical slices pre-labeled with [3H]cytidine or [3H]inositol in parallel were incubated with indicated concentrations of SKF38393 in the presence of 5 mM LiCl for 90 min, followed by assay of [3H]CDP-diacylglycerol (A), [3H]phosphoinositides (B) and [3H]inositol phosphates (C). Calculated ratios of CDP-diacylglycerol over inositol phosphates (CDP-DG/IP ratio), phosphatidylinositides (CDP-DG/PI ratio), or the sum of the inositol phosphates and phosphatidylinositides (CDP-DG/IPPI ratio) are shown in the right panel (D-E, respectively). Each bar is the mean ± SEM (n = 12). Data were analyzed by One-way ANOVA and posthoc Dunnett tests. #p < 0.05; *p < 0.01, compared to the respective control by Dunnett test.
Tyeryar et al. BMC Neuroscience 2008 9:12 doi:10.1186/1471-2202-9-12